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PDBsum entry 4lua
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References listed in PDB file
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Key reference
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Title
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Purification, Crystallization and preliminary X-Ray diffraction analysis of the n-Acetyltransferase sav0826 from staphylococcus aureus.
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Authors
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P.Srivastava,
Y.B.Khandokar,
J.K.Forwood.
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Ref.
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Acta Crystallogr F Struct Biol Commun, 2014,
70,
211-214.
[DOI no: ]
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PubMed id
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Abstract
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Staphylococcus aureus is a prevalent microorganism that is capable of causing a
wide range of infections and diseases. Several strains of this bacterial species
have developed antibiotic resistance to methicillin and vancomycin, and higher
death rates are still being reported each year owing to multidrug-resistant
strains. Certain GCN5-related N-acetyltransferases (GNATs) exhibit a broad
substrate range, including aminoglycosides, histones, other proteins and
serotonin, and have been implicated in antibiotic drug resistance. Here, the
expression, purification, crystallization and preliminary X-ray diffraction
analysis of a GNAT from S. aureus (SaNAT) are reported. SaNAT was recombinantly
expressed and crystallized by the hanging-drop vapour-diffusion method at 296 K,
and the crystals diffracted to 1.7 Å resolution on the MX2 beamline at the
Australian Synchrotron. The crystals belonged to space group P43212, with
unit-cell parameters a = b = 84.86, c = 49.06 Å, α = β = γ = 90°. A single
molecule is likely to be present in the asymmetric unit. A full structural and
functional analysis is currently being undertaken to provide novel insights into
the protein function, which in turn may provide a basis for drug design.
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