PDBsum entry 4lg2

RNA binding protein/RNA

PDB id: 4lg2

Contents

Protein chains

122 a.a.

DNA/RNA

Waters ×8

References listed in PDB file

Key reference

• Title: Ebolavirus vp35 coats the backbone of double-Stranded RNA for interferon antagonism.
• Authors: S.Bale, J.P.Julien, Z.A.Bornholdt, A.S.Krois, I.A.Wilson, E.O.Saphire.
• Ref.: J Virol, 2013, 87, 10385-10388. [DOI no: 10.1128/JVI.01452-13]
• PubMed id: 23824825

Abstract

Recognition of viral double-stranded RNA (dsRNA) activates interferon production and immune signaling in host cells. Crystal structures of ebolavirus VP35 show that it caps dsRNA ends to prevent sensing by pattern recognition receptors such as RIG-I. In contrast, structures of marburgvirus VP35 show that it primarily coats the dsRNA backbone. Here, we demonstrate that ebolavirus VP35 also coats the dsRNA backbone in solution, although binding to the dsRNA ends probably constitutes the initial binding event.