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PDBsum entry 4l85
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Transcription
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PDB id
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4l85
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Contents |
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116 a.a.
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115 a.a.
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120 a.a.
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References listed in PDB file
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Key reference
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Title
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An asymmetric heterodomain interface stabilizes a response regulator-Dna complex.
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Authors
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A.Narayanan,
S.Kumar,
A.N.Evrard,
L.N.Paul,
D.A.Yernool.
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Ref.
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Nat Commun, 2014,
5,
3282.
[DOI no: ]
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PubMed id
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Abstract
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Two-component signal transduction systems consist of pairs of histidine kinases
and response regulators, which mediate adaptive responses to environmental cues.
Most activated response regulators regulate transcription by binding tightly to
promoter DNA via a phosphorylation-triggered inactive-to-active transition. The
molecular basis for formation of stable response regulator-DNA complexes that
precede the assembly of RNA polymerases is unclear. Here, we present structures
of DNA complexed with the response regulator KdpE, a member of the OmpR/PhoB
family. The distinctively asymmetric complex in an active-like conformation
reveals a unique intramolecular interface between the receiver domain (RD) and
the DNA-binding domain (DBD) of only one of the two response regulators in the
complex. Structure-function studies show that this RD-DBD interface is necessary
to form stable complexes that support gene expression. The conservation of
sequence and structure suggests that these findings extend to a large group of
response regulators that act as transcription factors.
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