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PDBsum entry 4l73
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Metal transport
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PDB id
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4l73
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References listed in PDB file
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Key reference
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Title
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Structural basis of allosteric interactions among ca2+-Binding sites in a k+ channel rck domain.
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Authors
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F.J.Smith,
V.P.Pau,
G.Cingolani,
B.S.Rothberg.
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Ref.
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Nat Commun, 2013,
4,
2621.
[DOI no: ]
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PubMed id
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Abstract
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Ligand binding sites within proteins can interact by allosteric mechanisms to
modulate binding affinities and control protein function. Here we present
crystal structures of the regulator of K(+) conductance (RCK) domain from a K(+)
channel, MthK, which reveal the structural basis of allosteric coupling between
two Ca(2+) regulatory sites within the domain. Comparison of RCK domain crystal
structures in a range of conformations and with different numbers of regulatory
Ca(2+) ions bound, combined with complementary electrophysiological analysis of
channel gating, suggests chemical interactions that are important for modulation
of ligand binding and subsequent channel opening.
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