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UniProt functional annotation for P0A8Y8 | ||
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| UniProt code: P0A8Y8. |
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| Organism: | |
Escherichia coli (strain K12). |
| Taxonomy: | |
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia. |
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| Function: | |
Required for optimal enterobactin synthesis. Acts as a proofreading enzyme that prevents EntB misacylation by hydrolyzing the thioester bound existing between EntB and wrongly charged molecules. Displays esterase activity toward a wide range of substrates, including acyl-CoAs and aryl-CoAs. {ECO:0000269|PubMed:15808744, ECO:0000269|PubMed:17675380, ECO:0000269|PubMed:19119850, ECO:0000269|PubMed:19193103, ECO:0000269|PubMed:24992697}. |
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| Biophysicochemical properties: | |
Kinetic parameters: KM=16 uM for 2,3-DHB-EntB {ECO:0000269|PubMed:19119850}; KM=116 uM for 2,3-DHB-EntB {ECO:0000269|PubMed:19193103}; KM=21 uM for 4-hydroxybenzoyl-CoA {ECO:0000269|PubMed:19119850}; KM=190 uM for 4-hydroxybenzoyl-CoA {ECO:0000269|PubMed:19193103}; KM=25 uM for 2,4-DHB-EntB {ECO:0000269|PubMed:19119850}; KM=32 uM for lauroyl-EntB {ECO:0000269|PubMed:19119850}; KM=35 uM for 3-hydroxybenzoyl-CoA {ECO:0000269|PubMed:19119850}; KM=265 uM for 3-hydroxybenzoyl-CoA {ECO:0000269|PubMed:19193103}; KM=37 uM for 3-HPA-CoA {ECO:0000269|PubMed:19119850}; KM=45 uM for lauroyl-CoA {ECO:0000269|PubMed:19119850}; KM=49 uM for decanoyl-CoA {ECO:0000269|PubMed:19119850}; KM=55 uM for palmitoyl-CoA {ECO:0000269|PubMed:19119850}; KM=4.25 uM for palmitoyl-CoA {ECO:0000269|PubMed:19193103}; KM=161 uM for 2,3-dihydroxybenzoyl-CoA {ECO:0000269|PubMed:19193103}; KM=176 uM for salicylyl-CoA {ECO:0000269|PubMed:19193103}; KM=212 uM for 3,4-dihydroxybenzoyl-CoA {ECO:0000269|PubMed:19193103}; KM=219 uM for 2,4-dihydroxybenzoyl-CoA {ECO:0000269|PubMed:19193103}; KM=256 uM for 3,5-dihydroxybenzoyl-CoA {ECO:0000269|PubMed:19193103}; KM=272 uM for salicylyl-EntB {ECO:0000269|PubMed:19193103}; KM=350 uM for hexanoyl-CoA {ECO:0000269|PubMed:19119850}; KM=400 uM for propionyl-CoA {ECO:0000269|PubMed:19119850}; KM=475 uM for benzoyl-CoA {ECO:0000269|PubMed:19193103}; KM=800 uM for acetyl-CoA {ECO:0000269|PubMed:19119850}; |
| Pathway: | |
Siderophore biosynthesis; enterobactin biosynthesis. {ECO:0000255|HAMAP-Rule:MF_00907, ECO:0000269|PubMed:17675380}. |
| Subunit: | |
Homotetramer. Dimer of dimers. Interacts specifically with the aryl carrier protein (ArCP) domain of EntB. {ECO:0000255|HAMAP- Rule:MF_00907, ECO:0000269|PubMed:17675380, ECO:0000269|PubMed:19193103, ECO:0000269|PubMed:25010423}. |
| Subcellular location: | |
Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00907, ECO:0000269|PubMed:17675380}. |
| Induction: | |
Induced by iron starvation. {ECO:0000269|PubMed:17675380}. |
| Similarity: | |
Belongs to the thioesterase PaaI family. {ECO:0000255|HAMAP-Rule:MF_00907}. |
Annotations taken from UniProtKB at the EBI.