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UniProt functional annotation for O14214 | ||
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| UniProt code: O14214. |
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| Organism: | |
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast). |
| Taxonomy: | |
Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina; Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces. |
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| Function: | |
S-adenosyl-L-methionine-dependent guanine N(1)- methyltransferase that catalyzes the formation of N(1)-methylguanine at position 9 (m1G9) in cytoplasmic tRNA. {ECO:0000269|PubMed:24081582}. |
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| Catalytic activity: | |
Reaction=guanosine(9) in tRNA + S-adenosyl-L-methionine = H(+) + N(1)- methylguanosine(9) in tRNA + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:43156, Rhea:RHEA-COMP:10367, Rhea:RHEA-COMP:10368, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:73542, ChEBI:CHEBI:74269; EC=2.1.1.221; Evidence={ECO:0000269|PubMed:24081582}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=3.2 uM for tRNA(Gly) {ECO:0000269|PubMed:24081582}; Note=kcat is 0.16 min(-1) for tRNA(Gly) (m1G9)-methylation. {ECO:0000269|PubMed:24081582}; |
| Subunit: | |
Monomer. {ECO:0000269|PubMed:24081582}. |
| Subcellular location: | |
Cytoplasm {ECO:0000250|UniProtKB:Q12400}. Nucleus {ECO:0000250|UniProtKB:Q12400}. |
| Similarity: | |
Belongs to the class IV-like SAM-binding methyltransferase superfamily. TRM10 family. {ECO:0000255|PROSITE-ProRule:PRU01012}. |
Annotations taken from UniProtKB at the EBI.