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PDBsum entry 4jrt
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References listed in PDB file
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Key reference
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Title
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Structure of an a-Form RNA duplex obtained by degradation of 6s RNA in a crystallization droplet.
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Authors
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J.Kondo,
A.C.Dock-Bregeon,
D.K.Willkomm,
R.K.Hartmann,
E.Westhof.
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Ref.
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Acta Crystallogr Sect F Struct Biol Cryst Commun, 2013,
69,
634-639.
[DOI no: ]
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PubMed id
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Abstract
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In the course of a crystallographic study of a 132 nt variant of Aquifex
aeolicus 6S RNA, a crystal structure of an A-form RNA duplex containing 12 base
pairs was solved at a resolution of 2.6 Å. In fact, the RNA duplex is part of
the 6S RNA and was obtained by accidental but precise degradation of the 6S RNA
in a crystallization droplet. 6S RNA degradation was confirmed by microscopic
observation of crystals and gel electrophoresis of crystallization droplets. The
RNA oligomers obtained form regular A-form duplexes containing three GoU
wobble-type base pairs, one of which engages in intermolecular contacts through
a ribose-zipper motif at the crystal-packing interface.
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Headers
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