|
|
||
|
|
|
|
|
UniProt functional annotation for P9WMK5 | ||
|
|
|
|
|
|
||
| UniProt code: P9WMK5. |
|
||
| Organism: | |
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv). |
| Taxonomy: | |
Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. |
|
||
|
||
| Function: | |
Involved in cholesterol degradation. Catalyzes the retro- aldol cleavage of 4-hydroxy-2-oxohexanoate (HOHA) to pyruvate and propanal. Can also catalyze the cleavage of 4-hydroxy-2-oxopentanoate (HOPA) to pyruvate and acetaldehyde. The aldehydes produced by this reaction are directly channeled to the dehydrogenase HsaG. {ECO:0000269|PubMed:23614353}. |
|
||
| Catalytic activity: | |
Reaction=(S)-4-hydroxy-2-oxohexanoate = propanal + pyruvate; Xref=Rhea:RHEA:36003, ChEBI:CHEBI:15361, ChEBI:CHEBI:17153, ChEBI:CHEBI:73142; EC=4.1.3.43; Evidence={ECO:0000269|PubMed:23614353}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:36004; Evidence={ECO:0000269|PubMed:23614353}; |
| Catalytic activity: | |
Reaction=(S)-4-hydroxy-2-oxopentanoate = acetaldehyde + pyruvate; Xref=Rhea:RHEA:22624, ChEBI:CHEBI:15343, ChEBI:CHEBI:15361, ChEBI:CHEBI:73143; EC=4.1.3.39; Evidence={ECO:0000255|HAMAP- Rule:MF_01656, ECO:0000269|PubMed:23614353}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:22625; Evidence={ECO:0000269|PubMed:23614353}; |
| Cofactor: | |
Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000269|PubMed:23614353}; Name=Ni(2+); Xref=ChEBI:CHEBI:49786; Evidence={ECO:0000269|PubMed:23614353}; Name=Co(2+); Xref=ChEBI:CHEBI:48828; Evidence={ECO:0000269|PubMed:23614353}; Note=Requires a divalent metal cation. Activity is highest in the presence of Mn(2+), followed by Co(2+) and Ni(2+). Mg(2+) and Ca(2+) are poor metal cofactors. No activity with Cd(2+), Zn(2+) or Cu(2+). {ECO:0000269|PubMed:23614353}; |
| Activity regulation: | |
Shows aldolase activity only when expressed and copurified with HsaG. This arrangement probably prevents the deleterious formation and release of toxic aldehydes in the absence of the partner dehydrogenase. {ECO:0000269|PubMed:23614353}. |
| Biophysicochemical properties: | |
Kinetic parameters: KM=4.8 uM for 4-hydroxy-2-oxohexanoate {ECO:0000269|PubMed:23614353}; KM=4.4 uM for 4-hydroxy-2-oxopentanoate {ECO:0000269|PubMed:23614353}; Note=kcat is 0.38 sec(-1) with 4-hydroxy-2-oxohexanoate as substrate. kcat is 0.41 sec(-1) with 4-hydroxy-2-oxopentanoate as substrate. {ECO:0000269|PubMed:23614353}; |
| Subunit: | |
Homodimer. Forms an heterotetramer composed of two aldolase (HsaF) and two dehydrogenase (HsaG) subunits. {ECO:0000269|PubMed:23614353}. |
| Similarity: | |
Belongs to the 4-hydroxy-2-oxovalerate aldolase family. {ECO:0000255|HAMAP-Rule:MF_01656}. |
Annotations taken from UniProtKB at the EBI.