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PDBsum entry 4jkf
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RNA binding protein
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PDB id
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4jkf
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References listed in PDB file
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Key reference
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Title
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A conformational switch in prp8 mediates metal ion coordination that promotes pre-Mrna exon ligation.
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Authors
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M.J.Schellenberg,
T.Wu,
D.B.Ritchie,
S.Fica,
J.P.Staley,
K.A.Atta,
P.Lapointe,
A.M.Macmillan.
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Ref.
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Nat Struct Biol, 2013,
20,
728-734.
[DOI no: ]
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PubMed id
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Abstract
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Splicing of pre-mRNAs in eukaryotes is catalyzed by the spliceosome, a large
RNA-protein metalloenzyme. The catalytic center of the spliceosome involves a
structure comprising the U2 and U6 snRNAs and includes a metal bound by U6
snRNA. The precise architecture of the splicesome active site, however, and the
question of whether it includes protein components, remains unresolved. A wealth
of evidence places the protein PRP8 at the heart of the spliceosome through
assembly and catalysis. Here we provide evidence that the RNase H domain of PRP8
undergoes a conformational switch between the two steps of splicing,
rationalizing yeast prp8 alleles that promote either the first or second step.
We also show that this switch unmasks a metal-binding site involved in the
second step. Together, these data establish that PRP8 is a metalloprotein that
promotes exon ligation within the spliceosome.
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