 |
PDBsum entry 4jiz
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Signaling protein
|
PDB id
|
|
|
|
4jiz
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
References listed in PDB file
|
|
|
Key reference
|
|
|
Title
|
|
Activation of the yeast hippo pathway by phosphorylation-Dependent assembly of signaling complexes.
|
|
|
Authors
|
|
J.M.Rock,
D.Lim,
L.Stach,
R.W.Ogrodowicz,
J.M.Keck,
M.H.Jones,
C.C.Wong,
J.R.Yates,
M.Winey,
S.J.Smerdon,
M.B.Yaffe,
A.Amon.
|
|
|
Ref.
|
|
Science, 2013,
340,
871-875.
[DOI no: ]
|
|
|
PubMed id
|
|
|
|
|
|
Abstract
|
|
|
Scaffold-assisted signaling cascades guide cellular decision-making. In budding
yeast, one such signal transduction pathway called the mitotic exit network
(MEN) governs the transition from mitosis to the G1 phase of the cell cycle. The
MEN is conserved and in metazoans is known as the Hippo tumor-suppressor
pathway. We found that signaling through the MEN kinase cascade was mediated by
an unusual two-step process. The MEN kinase Cdc15 first phosphorylated the
scaffold Nud1. This created a phospho-docking site on Nud1, to which the
effector kinase complex Dbf2-Mob1 bound through a phosphoserine-threonine
binding domain, in order to be activated by Cdc15. This mechanism of pathway
activation has implications for signal transmission through other kinase
cascades and might represent a general principle in scaffold-assisted signaling.
|
|
|
|
|
|
|
