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UniProt functional annotation for P0A729 | ||
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| UniProt code: P0A729. |
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| Organism: | |
Escherichia coli (strain K12). |
| Taxonomy: | |
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia. |
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| Function: | |
Nucleoside triphosphate pyrophosphatase that hydrolyzes 7- methyl-GTP (m(7)GTP) (PubMed:24210219). May have a dual role in cell division arrest and in preventing the incorporation of modified nucleotides into cellular nucleic acids (PubMed:24210219). {ECO:0000269|PubMed:24210219}. |
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| Catalytic activity: | |
Reaction=H2O + N(7)-methyl-GTP = diphosphate + H(+) + N(7)-methyl-GMP; Xref=Rhea:RHEA:58744, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:33019, ChEBI:CHEBI:58285, ChEBI:CHEBI:87133; Evidence={ECO:0000255|HAMAP-Rule:MF_00528, ECO:0000269|PubMed:24210219}; |
| Cofactor: | |
Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240; Evidence={ECO:0000255|HAMAP-Rule:MF_00528}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=32.8 uM for m(7)GTP {ECO:0000269|PubMed:24210219}; Note=kcat is 1.2 sec(-1). {ECO:0000269|PubMed:24210219}; |
| Subunit: | |
Monomer and homodimer. {ECO:0000269|PubMed:24210219}. |
| Subcellular location: | |
Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00528, ECO:0000305}. |
| Similarity: | |
Belongs to the Maf family. YceF subfamily. {ECO:0000255|HAMAP-Rule:MF_00528, ECO:0000305|PubMed:24210219}. |
| Sequence caution: | |
Sequence=AAA23829.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305}; |
Annotations taken from UniProtKB at the EBI.