 |
PDBsum entry 4jb9
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Viral protein/immune system
|
PDB id
|
|
|
|
4jb9
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
Contents |
 |
|
|
|
|
|
|
|
|
|
|
|
344 a.a.
|
|
|
|
|
|
|
|
|
|
|
225 a.a.
|
|
|
|
|
|
|
|
|
|
|
209 a.a.
|
|
|
|
|
|
|
|
|
|
|
|
|
|
References listed in PDB file
|
|
|
Key reference
|
|
|
Title
|
|
Delineating antibody recognition in polyclonal sera from patterns of HIV-1 isolate neutralization.
|
|
|
Authors
|
|
I.S.Georgiev,
N.A.Doria-Rose,
T.Zhou,
Y.D.Kwon,
R.P.Staupe,
S.Moquin,
G.Y.Chuang,
M.K.Louder,
S.D.Schmidt,
H.R.Altae-Tran,
R.T.Bailer,
K.Mckee,
M.Nason,
S.O'Dell,
G.Ofek,
M.Pancera,
S.Srivatsan,
L.Shapiro,
M.Connors,
S.A.Migueles,
L.Morris,
Y.Nishimura,
M.A.Martin,
J.R.Mascola,
P.D.Kwong.
|
|
|
Ref.
|
|
Science, 2013,
340,
751-756.
[DOI no: ]
|
|
|
PubMed id
|
|
|
|
|
|
Abstract
|
|
|
Serum characterization and antibody isolation are transforming our understanding
of the humoral immune response to viral infection. Here, we show that epitope
specificities of HIV-1-neutralizing antibodies in serum can be elucidated from
the serum pattern of neutralization against a diverse panel of HIV-1 isolates.
We determined "neutralization fingerprints" for 30 neutralizing
antibodies on a panel of 34 diverse HIV-1 strains and showed that similarity in
neutralization fingerprint correlated with similarity in epitope. We used these
fingerprints to delineate specificities of polyclonal sera from 24
HIV-1-infected donors and a chimeric siman-human immunodeficiency virus-infected
macaque. Delineated specificities matched published specificities and were
further confirmed by antibody isolation for two sera. Patterns of virus-isolate
neutralization can thus afford a detailed epitope-specific understanding of
neutralizing-antibody responses to viral infection.
|
|
|
|
|
|
|
