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UniProt functional annotation for P53008 | ||
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| UniProt code: P53008. |
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| Organism: | |
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). |
| Taxonomy: | |
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces. |
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| Function: | |
Cleaves the distal alpha 1,2-linked glucose residue from the Glc(3)Man(9)GlcNAc(2) oligosaccharide precursor highly specifically (PubMed:9363442). Seems to play a role in beta-1,6-glucan synthesis (PubMed:8576053). {ECO:0000269|PubMed:8576053, ECO:0000269|PubMed:9363442}. |
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| Catalytic activity: | |
Reaction=H2O + N(4)-(alpha-D-Glc-(1->2)-alpha-D-Glc-(1->3)-alpha-D-Glc- (1->3)-alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)- [alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D- Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc- (1->4)-alpha-D-GlcNAc)-L-asparaginyl-[protein] = beta-D-glucose + N(4)-(alpha-D-Glc-(1->3)-alpha-D-Glc-(1->3)-alpha-D-Man-(1->2)-alpha- D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man- (1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]- beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc)-L-asparaginyl- [protein]; Xref=Rhea:RHEA:55988, Rhea:RHEA-COMP:12806, Rhea:RHEA- COMP:14355, ChEBI:CHEBI:15377, ChEBI:CHEBI:15903, ChEBI:CHEBI:59082, ChEBI:CHEBI:132537; EC=3.2.1.106; Evidence={ECO:0000269|PubMed:23536181}; |
| Activity regulation: | |
Miglitol is an effective inhibitor at 1 mM. {ECO:0000269|PubMed:23536181}. |
| Pathway: | |
Glycan metabolism; N-glycan degradation. {ECO:0000305}. |
| Subcellular location: | |
Endoplasmic reticulum membrane {ECO:0000269|PubMed:8576053}; Single-pass type II membrane protein {ECO:0000269|PubMed:8576053}. |
| Ptm: | |
N-glycosylated. {ECO:0000269|PubMed:8576053}. |
| Disruption phenotype: | |
Displays phenotypes characteristic of cell wall defects such as hypersensitivity to calcofluor white and resistance to K1 killer toxin, and results in a 50% reduction of cell wall beta-1,6- glucan level (PubMed:9363442). Abolishes glucosidase I activity (PubMed:9363442). {ECO:0000269|PubMed:8576053, ECO:0000269|PubMed:9363442}. |
| Miscellaneous: | |
Present with 2840 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}. |
| Similarity: | |
Belongs to the glycosyl hydrolase 63 family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.