PDBsum entry 4j4q

Signaling protein

PDB id: 4j4q

Contents

Protein chains

326 a.a.

11 a.a.

Ligands

NAG-NAG-BMA-MAN

GLC-GLC

BOG ×4

PLM

SO4 ×2

ACT

Waters ×32

PDB id:

4j4q

Name:

Signaling protein

Title:

Crystal structure of active conformation of gpcr opsin stabilized by octylglucoside

Structure:

Rhodopsin. Chain: a. Guanine nucleotide-binding protein g(t) subunit alpha-1. Chain: b. Fragment: c-terminal derived peptide, unp residues 340-350. Synonym: transducin alpha-1 chain. Engineered: yes. Mutation: yes

Source:

Bos taurus. Bovine. Organism_taxid: 9913. Strain: bovine. Synthetic: yes. Other_details: this peptide was chemically synthesized with k341l and c347v mutation

Resolution:

2.65Å R-factor: 0.218 R-free: 0.230

Authors:

J.H.Park,T.Morizumi,Y.Li,J.E.Hong,E.F.Pai,K.P.Hofmann,H.W.Choe, O.P.Ernst

Key ref:

J.H.Park et al. (2013). Opsin, a structural model for olfactory receptors? Angew Chem Int Ed Engl, 52, 11021-11024. PubMed id: 24038729 DOI: 10.1002/anie.201302374

Date:

07-Feb-13 Release date: 30-Oct-13

Protein chain

P02699  (OPSD_BOVIN) -  Rhodopsin from Bos taurus

Seq: 348 a.a.

Struc: 326 a.a.

Protein chain

P04695  (GNAT1_BOVIN) -  Guanine nucleotide-binding protein G(t) subunit alpha-1 from Bos taurus

Seq: 350 a.a.

Struc: 11 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

DOI no: 10.1002/anie.201302374

Angew Chem Int Ed Engl 52:11021-11024 (2013)

PubMed id: 24038729

Opsin, a structural model for olfactory receptors?

J.H.Park, T.Morizumi, Y.Li, J.E.Hong, E.F.Pai, K.P.Hofmann, H.W.Choe, O.P.Ernst.

ABSTRACT

Receptor-ligand interaction: Olfactory receptors (ORs) are G-protein-coupled receptors (GPCRs), which detect signaling molecules such as hormones and odorants. The structure of opsin, the GPCR employed in vision, with a detergent molecule bound deep in its orthosteric ligand-binding pocket provides a template for OR homology modeling, thus enabling investigation of the structural basis of the mechanism of odorant-receptor recognition.