UniProt functional annotation for D4HRL0



	UniProt functional annotation for D4HRL0

UniProt code: D4HRL0.

Organism: Limnoria quadripunctata (Gribble).

Taxonomy: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Crustacea; Multicrustacea; Malacostraca; Eumalacostraca; Peracarida; Isopoda; Limnoriidae; Limnoria.

Function: Exocellobiohydrolase (CBH) that catalyzes the hydrolysis of 1,4-beta-D-glucosidic bonds in cellulose to release the disaccharide cellobiose (PubMed:23733951). The degradation of cellulose involves an interplay between different cellulolytic enzymes. Hydrolysis starts with endoglucanases (EGs), which cut internal beta-1,4-glucosidic bonds in cellulose to reduce the polymerization degree of the substrate and create new chain ends for exocellobiohydrolases (CBHs). The CBHs release the disaccharide cellobiose from the non-reducing end of the cellulose polymer chain. Finally, beta-1,4-glucosidases hydrolyze the cellobiose and other short cello-oligosaccharides into glucose units (Probable). {ECO:0000269|PubMed:23733951, ECO:0000305}.

Catalytic activity: Reaction=Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains.; EC=3.2.1.91; Evidence={ECO:0000269|PubMed:23733951};

Biophysicochemical properties: Kinetic parameters: KM=2676.4 uM for p-nitrophenyl-beta-d-cellotrioside (pNP-G3) {ECO:0000269|PubMed:23733951}; KM=2121.7 uM for p-nitrophenyl-beta-d-cellopentaoside (pNP-G5) {ECO:0000269|PubMed:23733951}; Note=kcat is 10.57 min(-1) for p-nitrophenyl-beta-d-cellotrioside (pNP-G3). kcat is 24.42 min(-1) for p-nitrophenyl-beta-d- cellopentaoside (pNP-G5). {ECO:0000269|PubMed:23733951}; pH dependence: Optimum pH is 4-6.5 for p-nitrophenyl-beta-d-cellotrioside (pNP-G3), and 4-8 for p-nitrophenyl-beta-d-cellopentaoside (pNP-G5). {ECO:0000269|PubMed:23733951}; Temperature dependence: Optimum temperature is approximately 25-45 degrees Celsius. High activity is maintained over a wide temperature range. {ECO:0000269|PubMed:23733951};

Subunit: Monomer. {ECO:0000269|PubMed:23733951}.

Subcellular location: Secreted {ECO:0000305}.

Tissue specificity: Highly expressed in the hepatopancreas (at protein level) (PubMed:20212162, PubMed:23733951). Little or no expression detected in the hindgut or the rest of the body (at protein level) (PubMed:23733951). {ECO:0000269|PubMed:20212162, ECO:0000269|PubMed:23733951}.

Miscellaneous: Unlike most wood-boring animal species, which rely on symbiotic microbes for digestion of woody substrates, the L.quadripunctata genome contains a range of enzymes (including GH7B) capable of lignocellulose digestion. {ECO:0000269|PubMed:20212162}.

Similarity: Belongs to the glycosyl hydrolase 7 (cellulase C) family. {ECO:0000305}.

Annotations taken from UniProtKB at the EBI.


Organism:		Limnoria quadripunctata (Gribble).
Taxonomy:		Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Crustacea; Multicrustacea; Malacostraca; Eumalacostraca; Peracarida; Isopoda; Limnoriidae; Limnoria.



Function:		Exocellobiohydrolase (CBH) that catalyzes the hydrolysis of 1,4-beta-D-glucosidic bonds in cellulose to release the disaccharide cellobiose (PubMed:23733951). The degradation of cellulose involves an interplay between different cellulolytic enzymes. Hydrolysis starts with endoglucanases (EGs), which cut internal beta-1,4-glucosidic bonds in cellulose to reduce the polymerization degree of the substrate and create new chain ends for exocellobiohydrolases (CBHs). The CBHs release the disaccharide cellobiose from the non-reducing end of the cellulose polymer chain. Finally, beta-1,4-glucosidases hydrolyze the cellobiose and other short cello-oligosaccharides into glucose units (Probable). {ECO:0000269\|PubMed:23733951, ECO:0000305}.


Catalytic activity:		Reaction=Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains.; EC=3.2.1.91; Evidence={ECO:0000269\|PubMed:23733951};
Biophysicochemical properties:		Kinetic parameters: KM=2676.4 uM for p-nitrophenyl-beta-d-cellotrioside (pNP-G3) {ECO:0000269\|PubMed:23733951}; KM=2121.7 uM for p-nitrophenyl-beta-d-cellopentaoside (pNP-G5) {ECO:0000269\|PubMed:23733951}; Note=kcat is 10.57 min(-1) for p-nitrophenyl-beta-d-cellotrioside (pNP-G3). kcat is 24.42 min(-1) for p-nitrophenyl-beta-d- cellopentaoside (pNP-G5). {ECO:0000269\|PubMed:23733951}; pH dependence: Optimum pH is 4-6.5 for p-nitrophenyl-beta-d-cellotrioside (pNP-G3), and 4-8 for p-nitrophenyl-beta-d-cellopentaoside (pNP-G5). {ECO:0000269\|PubMed:23733951}; Temperature dependence: Optimum temperature is approximately 25-45 degrees Celsius. High activity is maintained over a wide temperature range. {ECO:0000269\|PubMed:23733951};
Subunit:		Monomer. {ECO:0000269\|PubMed:23733951}.
Subcellular location:		Secreted {ECO:0000305}.
Tissue specificity:		Highly expressed in the hepatopancreas (at protein level) (PubMed:20212162, PubMed:23733951). Little or no expression detected in the hindgut or the rest of the body (at protein level) (PubMed:23733951). {ECO:0000269\|PubMed:20212162, ECO:0000269\|PubMed:23733951}.
Miscellaneous:		Unlike most wood-boring animal species, which rely on symbiotic microbes for digestion of woody substrates, the L.quadripunctata genome contains a range of enzymes (including GH7B) capable of lignocellulose digestion. {ECO:0000269\|PubMed:20212162}.
Similarity:		Belongs to the glycosyl hydrolase 7 (cellulase C) family. {ECO:0000305}.