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PDBsum entry 4i3c
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Fluorescent protein
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PDB id
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4i3c
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References listed in PDB file
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Key reference
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Title
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A bilirubin-Inducible fluorescent protein from eel muscle.
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Authors
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A.Kumagai,
R.Ando,
H.Miyatake,
P.Greimel,
T.Kobayashi,
Y.Hirabayashi,
T.Shimogori,
A.Miyawaki.
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Ref.
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Cell, 2013,
153,
1602-1611.
[DOI no: ]
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PubMed id
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Abstract
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The fluorescent protein toolbox has revolutionized experimental biology. Despite
this advance, no fluorescent proteins have been identified from vertebrates, nor
has chromogenic ligand-inducible activation or clinical utility been
demonstrated. Here, we report the cloning and characterization of UnaG, a
fluorescent protein from Japanese eel. UnaG belongs to the fatty-acid-binding
protein (FABP) family, and expression in eel is restricted to small-diameter
muscle fibers. On heterologous expression in cell lines or mouse brain, UnaG
produces oxygen-independent green fluorescence. Remarkably, UnaG fluorescence is
triggered by an endogenous ligand, bilirubin, a membrane-permeable heme
metabolite and clinical health biomarker. The holoUnaG structure at 1.2 Å
revealed a biplanar coordination of bilirubin by reversible π-conjugation, and
we used this high-affinity and high-specificity interaction to establish a
fluorescence-based human bilirubin assay with promising clinical utility. UnaG
will be the prototype for a versatile class of ligand-activated fluorescent
proteins, with applications in research, medicine, and bioengineering.
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