PDBsum entry 4htt

Hydrolase

PDB id: 4htt

Contents

Protein chains

225 a.a.

References listed in PDB file

Key reference

• Title: The glove-Like structure of the conserved membrane protein tatc provides insight into signal sequence recognition in twin-Arginine translocation.
• Authors: S.Ramasamy, R.Abrol, C.J.Suloway, W.M.Clemons.
• Ref.: Structure, 2013, 21, 777-788. [DOI no: 10.1016/j.str.2013.03.004]
• PubMed id: 23583035

Abstract

In bacteria, two signal-sequence-dependent secretion pathways translocate proteins across the cytoplasmic membrane. Although the mechanism of the ubiquitous general secretory pathway is becoming well understood, that of the twin-arginine translocation pathway, responsible for translocation of folded proteins across the bilayer, is more mysterious. TatC, the largest and most conserved of three integral membrane components, provides the initial binding site of the signal sequence prior to pore assembly. Here, we present two crystal structures of TatC from the thermophilic bacteria Aquifex aeolicus at 4.0 Å and 6.8 Å resolution. The membrane architecture of TatC includes a glove-shaped structure with a lipid-exposed pocket predicted by molecular dynamics to distort the membrane. Correlating the biochemical literature to these results suggests that the signal sequence binds in this pocket, leading to structural changes that facilitate higher order assemblies.