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PDBsum entry 4hq9
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Fluorescent protein
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PDB id
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4hq9
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References listed in PDB file
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Key reference
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Title
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Green-To-Red photoconvertible dronpa mutant for multimodal super-Resolution fluorescence microscopy.
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Authors
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B.Moeyaert,
N.Nguyen bich,
E.De zitter,
S.Rocha,
K.Clays,
H.Mizuno,
L.Van meervelt,
J.Hofkens,
P.Dedecker.
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Ref.
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Acs Nano, 2014,
8,
1664-1673.
[DOI no: ]
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PubMed id
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Abstract
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Advanced imaging techniques crucially depend on the labels used. In this work,
we present the structure-guided design of a fluorescent protein that displays
both reversibly photochromic and green-to-red photoconversion behavior. We first
designed ffDronpa, a mutant of the photochromic fluorescent protein Dronpa that
matures up to three times faster while retaining its interesting photochromic
features. Using a combined evolutionary and structure-driven rational design
strategy, we developed a green-to-red photoconvertible ffDronpa mutant, called
pcDronpa, and explored different optimization strategies that resulted in its
improved version, pcDronpa2. This fluorescent probe combines a high brightness
with low photobleaching and photoblinking. We herein show that, despite its
tetrameric nature, pcDronpa2 allows for multimodal subdiffraction imaging by
sequentially imaging a given sample using both super-resolution fluctuation
imaging and localization microscopy.
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