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UniProt functional annotation for Q9ALJ4 | ||
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| UniProt code: Q9ALJ4. |
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| Organism: | |
Geobacillus stearothermophilus (Bacillus stearothermophilus). |
| Taxonomy: | |
Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Geobacillus. |
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| Function: | |
Hydrolyzes the short-chain alpha-galactosaccharides raffinose and stachyose. {ECO:0000269|PubMed:23012371}. |
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| Catalytic activity: | |
Reaction=Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids.; EC=3.2.1.22; Evidence={ECO:0000269|PubMed:16511274, ECO:0000269|PubMed:23012371, ECO:0000269|Ref.1}; |
| Activity regulation: | |
Not inhibited by D-galactose or sucrose (Ref.1). Inhibited by pharmaceutical drug 1-deoxygalactonojirimycin (PubMed:23012371). {ECO:0000269|PubMed:23012371, ECO:0000269|Ref.1}. |
| Biophysicochemical properties: | |
Kinetic parameters: KM=0.21 mM for p-nitrophenyl-alpha-galactopyranoside (at 25 degrees Celsius and pH 6.5) {ECO:0000269|PubMed:23012371}; KM=3.8 mM for raffinose (at 25 degrees Celsius and pH 6.5) {ECO:0000269|PubMed:23012371}; Note=kcat is 105 s(-1) for p-nitrophenyl-alpha-galactopyranoside (at 25 degrees Celsius and pH 6.5). kcat is 180 s(-1) for raffinose (at 25 degrees Celsius and pH 6.5). {ECO:0000269|PubMed:23012371}; pH dependence: Optimum pH is 6.5. {ECO:0000269|Ref.1}; Temperature dependence: Optimum temperature is 65 degrees Celsius. {ECO:0000269|Ref.1}; |
| Subunit: | |
Homotetramer. {ECO:0000250|UniProtKB:G1UB44}. |
| Biotechnology: | |
Has properties that make it suitable for elimination of D-raffinose from low green syrup in sugar manufacturing process from beets in order to improve the yield of sucrose. {ECO:0000269|Ref.1}. |
| Similarity: | |
Belongs to the glycosyl hydrolase 36 family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.