UniProt functional annotation for P32300



	UniProt functional annotation for P32300

UniProt code: P32300.

Organism: Mus musculus (Mouse).

Taxonomy: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus.

Function: G-protein coupled receptor that functions as receptor for endogenous enkephalins and for a subset of other opioids. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling leads to the inhibition of adenylate cyclase activity. Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Plays a role in the perception of pain and in opiate-mediated analgesia. Plays a role in developing analgesic tolerance to morphine. {ECO:0000269|PubMed:10677041, ECO:0000269|PubMed:1334555, ECO:0000269|PubMed:1335167}.

Subunit: May form homooligomers. Forms a heterodimer with OPRM1 (PubMed:18836069). Interacts with GPRASP1 (By similarity). Interacts with RTP4; the interaction promotes cell surface localization of the OPRD1-OPRM1 heterodimer (PubMed:18836069). {ECO:0000250|UniProtKB:P41143, ECO:0000269|PubMed:18836069}.

Subcellular location: Cell membrane {ECO:0000269|PubMed:10677041, ECO:0000269|PubMed:1334555, ECO:0000269|PubMed:1335167, ECO:0000269|PubMed:18836069, ECO:0000269|PubMed:22596164}; Multi-pass membrane protein {ECO:0000269|PubMed:10677041, ECO:0000269|PubMed:1334555, ECO:0000269|PubMed:1335167, ECO:0000269|PubMed:22596164}.

Tissue specificity: Brain, with high concentrations in the basal ganglia and limbic regions. {ECO:0000269|PubMed:10677041, ECO:0000269|PubMed:1335167}.

Ptm: Ubiquitinated. A basal ubiquitination seems not to be related to degradation. Ubiquitination is increased upon formation of OPRM1:OPRD1 oligomers leading to proteasomal degradation; the ubiquitination is diminished by RTP4. {ECO:0000269|PubMed:18836069}.

Disruption phenotype: Mice are born at the expected Mendelian rate; they show no obvious phenotype and are fertile. Mutant mice show decreased analgesia in response to opioids, such as deltorphin-2. They do not develop analgesic tolerance to morphine. {ECO:0000269|PubMed:10677041}.

Similarity: Belongs to the G-protein coupled receptor 1 family. {ECO:0000255|PROSITE-ProRule:PRU00521}.

Annotations taken from UniProtKB at the EBI.


Organism:		Mus musculus (Mouse).
Taxonomy:		Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus.



Function:		G-protein coupled receptor that functions as receptor for endogenous enkephalins and for a subset of other opioids. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling leads to the inhibition of adenylate cyclase activity. Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Plays a role in the perception of pain and in opiate-mediated analgesia. Plays a role in developing analgesic tolerance to morphine. {ECO:0000269\|PubMed:10677041, ECO:0000269\|PubMed:1334555, ECO:0000269\|PubMed:1335167}.


Subunit:		May form homooligomers. Forms a heterodimer with OPRM1 (PubMed:18836069). Interacts with GPRASP1 (By similarity). Interacts with RTP4; the interaction promotes cell surface localization of the OPRD1-OPRM1 heterodimer (PubMed:18836069). {ECO:0000250\|UniProtKB:P41143, ECO:0000269\|PubMed:18836069}.
Subcellular location:		Cell membrane {ECO:0000269\|PubMed:10677041, ECO:0000269\|PubMed:1334555, ECO:0000269\|PubMed:1335167, ECO:0000269\|PubMed:18836069, ECO:0000269\|PubMed:22596164}; Multi-pass membrane protein {ECO:0000269\|PubMed:10677041, ECO:0000269\|PubMed:1334555, ECO:0000269\|PubMed:1335167, ECO:0000269\|PubMed:22596164}.
Tissue specificity:		Brain, with high concentrations in the basal ganglia and limbic regions. {ECO:0000269\|PubMed:10677041, ECO:0000269\|PubMed:1335167}.
Ptm:		Ubiquitinated. A basal ubiquitination seems not to be related to degradation. Ubiquitination is increased upon formation of OPRM1:OPRD1 oligomers leading to proteasomal degradation; the ubiquitination is diminished by RTP4. {ECO:0000269\|PubMed:18836069}.
Disruption phenotype:		Mice are born at the expected Mendelian rate; they show no obvious phenotype and are fertile. Mutant mice show decreased analgesia in response to opioids, such as deltorphin-2. They do not develop analgesic tolerance to morphine. {ECO:0000269\|PubMed:10677041}.
Similarity:		Belongs to the G-protein coupled receptor 1 family. {ECO:0000255\|PROSITE-ProRule:PRU00521}.