 |
PDBsum entry 4e1v
|
|
|
|
References listed in PDB file
|
 |
|
Key reference
|
|
|
Title
|
|
X-Ray structure of salmonella typhimurium uridine phosphorylase complexed with 5-Fluorouracil and molecular modelling of the complex of 5-Fluorouracil with uridine phosphorylase from vibrio cholerae.
|
|
|
Authors
|
|
A.A.Lashkov,
S.E.Sotnichenko,
I.I.Prokofiev,
A.G.Gabdulkhakov,
I.I.Agapov,
A.A.Shtil,
C.Betzel,
A.S.Mironov,
A.M.Mikhailov.
|
|
|
Ref.
|
|
Acta Crystallogr D Biol Crystallogr, 2012,
68,
968-974.
|
|
|
PubMed id
|
|
|
|
|
|
Abstract
|
|
|
Uridine phosphorylase (UPh), which is a key enzyme in the reutilization pathway
of pyrimidine nucleoside metabolism, is a validated target for the treatment of
infectious diseases and cancer. A detailed analysis of the interactions of UPh
with the therapeutic ligand 5-fluorouracil (5-FUra) is important for the
rational design of pharmacological inhibitors of these enzymes in prokaryotes
and eukaryotes. Expanding on the preliminary analysis of the spatial
organization of the active centre of UPh from the pathogenic bacterium
Salmonella typhimurium (StUPh) in complex with 5-FUra [Lashkov et al. (2009),
Acta Cryst. F65, 601-603], the X-ray structure of the StUPh-5-FUra complex was
analysed at atomic resolution and an in silico model of the complex formed by
the drug with UPh from Vibrio cholerae (VchUPh) was generated. These results
should be considered in the design of selective inhibitors of UPhs from various
species.
|
|
|
Secondary reference #1
|
|
|
Title
|
|
Crystallization and preliminary X-Ray diffraction analysis of salmonella typhimurium uridine phosphorylase complexed with 5-Fluorouracil.
|
|
|
Authors
|
|
A.A.Lashkov,
A.G.Gabdoulkhakov,
A.A.Shtil,
A.M.Mikhailov.
|
|
|
Ref.
|
|
Acta Crystallogr Sect F Struct Biol Cryst Commun, 2009,
65,
601-603.
|
|
|
PubMed id
|
|
|
|
|
|
|
|
|
|
|
|
|
