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PDBsum entry 4d4u
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Sugar binding protein
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PDB id
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4d4u
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References listed in PDB file
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Key reference
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Title
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Structural insights into aspergillus fumigatus lectin specificity: afl binding sites are functionally non-Equivalent.
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Authors
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J.Houser,
J.Komarek,
G.Cioci,
A.Varrot,
A.Imberty,
M.Wimmerova.
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Ref.
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Acta Crystallogr D Biol Crystallogr, 2015,
71,
442-453.
[DOI no: ]
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PubMed id
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Abstract
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The Aspergillus fumigatus lectin AFL was recently described as a new member of
the AAL lectin family. As a lectin from an opportunistic pathogen, it might play
an important role in the interaction of the pathogen with the human host. A
detailed study of structures of AFL complexed with several monosaccharides and
oligosaccharides, including blood-group epitopes, was combined with affinity
data from SPR and discussed in the context of previous findings. Its six binding
sites are non-equivalent, and owing to minor differences in amino-acid
composition they exhibit a marked difference in specific ligand recognition. AFL
displays a high affinity in the micromolar range towards oligosaccharides which
were detected in plants and also those bound on the human epithelia. All of
these results indicate AFL to be a complex member of the lectin family and a
challenging target for future medical research and, owing to its binding
properties, a potentially useful tool in specific biotechnological applications.
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