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UniProt functional annotation for Q9HTJ1 | ||
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| UniProt code: Q9HTJ1. |
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| Organism: | |
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1). |
| Taxonomy: | |
Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas. |
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| Function: | |
Involved in the biosynthesis of the osmoprotectant glycine betaine. Catalyzes the irreversible oxidation of betaine aldehyde to the corresponding acid. In P.aeruginosa this reaction is a compulsory step in the assimilation of carbon and nitrogen when bacteria are growing in choline or choline precursors. Can use NADP(+) with similar efficiency to NAD(+), a property that can be used by the bacterium to produce the NADPH needed to combat the oxidative stress imposed by the host defenses. {ECO:0000255|HAMAP-Rule:MF_00804, ECO:0000269|PubMed:11104673, ECO:0000269|PubMed:21732915}. |
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| Catalytic activity: | |
Reaction=betaine aldehyde + H2O + NAD(+) = glycine betaine + 2 H(+) + NADH; Xref=Rhea:RHEA:15305, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15710, ChEBI:CHEBI:17750, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.2.1.8; Evidence={ECO:0000255|HAMAP- Rule:MF_00804, ECO:0000269|PubMed:11104673}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:15306; Evidence={ECO:0000305|PubMed:11104673}; |
| Catalytic activity: | |
Reaction=betaine aldehyde + H2O + NADP(+) = glycine betaine + 2 H(+) + NADPH; Xref=Rhea:RHEA:30067, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15710, ChEBI:CHEBI:17750, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; Evidence={ECO:0000269|PubMed:11104673}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:30068; Evidence={ECO:0000305|PubMed:11104673}; |
| Cofactor: | |
Name=K(+); Xref=ChEBI:CHEBI:29103; Evidence={ECO:0000255|HAMAP-Rule:MF_00804, ECO:0000269|PubMed:19013472, ECO:0000269|PubMed:21732915}; Note=Binds 2 potassium ions per subunit. {ECO:0000255|HAMAP- Rule:MF_00804, ECO:0000269|PubMed:19013472, ECO:0000269|PubMed:21732915}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=507 uM for betaine aldehyde (at pH 8.0 and 30 degrees Celsius, in the presence of NADP(+)) {ECO:0000269|PubMed:11104673}; KM=434 uM for betaine aldehyde (at pH 8.0 and 30 degrees Celsius, in the presence of NAD(+)) {ECO:0000269|PubMed:11104673}; KM=83 uM for NADP(+) (at pH 8.0 and 30 degrees Celsius) {ECO:0000269|PubMed:11104673}; KM=385 uM for NAD(+) (at pH 8.0 and 30 degrees Celsius) {ECO:0000269|PubMed:11104673}; Note=kcat is 261 sec(-1) for the NADP-dependent oxidation of betaine aldehyde. kcat is 276 sec(-1) for the NAD-dependent oxidation of betaine aldehyde. {ECO:0000269|PubMed:11104673}; |
| Pathway: | |
Amine and polyamine biosynthesis; betaine biosynthesis via choline pathway; betaine from betaine aldehyde: step 1/1. {ECO:0000255|HAMAP-Rule:MF_00804}. |
| Subunit: | |
Dimer of dimers. {ECO:0000255|HAMAP-Rule:MF_00804, ECO:0000269|PubMed:19013472, ECO:0000269|PubMed:21732915, ECO:0000269|Ref.4}. |
| Similarity: | |
Belongs to the aldehyde dehydrogenase family. {ECO:0000255|HAMAP-Rule:MF_00804}. |
Annotations taken from UniProtKB at the EBI.