UniProt functional annotation for P97287



	UniProt functional annotation for P97287

UniProt code: P97287.

Organism: Mus musculus (Mouse).

Taxonomy: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus.

Function: Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 2 has antiapoptotic activity. {ECO:0000269|PubMed:16543145, ECO:0000269|PubMed:19919825}.

Subunit: Interacts with HIF3A isoform 2 (via C-terminus domain) (PubMed:21546903). Interacts with BAD, BOK, BIK, BAX, BAK1, and TPT1. Interacts with BBC3, BMF and PMAIP1 (PubMed:15550399, PubMed:18589438). Interacts with BOP. Interacts with BCL2L11; this interaction may sequester BCL2L11 and prevent its pro-apoptotic activity (PubMed:16543145, PubMed:27013495, PubMed:15550399). Interacts with GIMAP5 and HSPA8/HSC70; the interaction between HSPA8 and MCL1 is impaired in the absence of GIMAP5 (PubMed:21502331). {ECO:0000269|PubMed:15550399, ECO:0000269|PubMed:16543145, ECO:0000269|PubMed:18589438, ECO:0000269|PubMed:21502331, ECO:0000269|PubMed:21546903, ECO:0000269|PubMed:27013495}.

Subcellular location: Membrane {ECO:0000250}; Single-pass membrane protein {ECO:0000250}. Cytoplasm {ECO:0000250}. Mitochondrion. Nucleus, nucleoplasm {ECO:0000250}. Note=Cytoplasmic, associated with mitochondria.

Induction: Up-regulated by IL3 and CSF2. Up-regulated in murine embryonal carcinoma cells in response to retinoic acid treatment. Levels reach a maximum after 4 hours, are decreased after 8 hours and are back to maximum after 12 hours. Levels are decreased after 24 hours and back to basal levels after 48 hours. Expression remains constant in retinoic acid-treated embryonic stem cells. {ECO:0000269|PubMed:9655929, ECO:0000269|PubMed:9671497}.

Ptm: Cleaved by CASP3 during apoptosis, yielding a pro-apoptotic C- terminal fragment. {ECO:0000250}.

Ptm: Rapidly degraded in the absence of phosphorylation in the PEST region. {ECO:0000250}.

Ptm: Phosphorylated on Ser-140, by GSK3, in response to IL3/interleukin-3 withdrawal. Phosphorylation at Ser-140 induces ubiquitination and proteasomal degradation, abrogating the anti- apoptotic activity. Treatment with taxol or okadaic acid induces phosphorylation on additional sites. {ECO:0000269|PubMed:16543145}.

Ptm: Ubiquitinated. Ubiquitination is induced by phosphorylation at Ser-140 (By similarity). {ECO:0000250}.

Miscellaneous: [Isoform 2]: This isoform is more stable than isoform 1 in cells undergoing apoptosis. {ECO:0000305}.

Similarity: Belongs to the Bcl-2 family. {ECO:0000305}.

Annotations taken from UniProtKB at the EBI.


Organism:		Mus musculus (Mouse).
Taxonomy:		Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus.



Function:		Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 2 has antiapoptotic activity. {ECO:0000269\|PubMed:16543145, ECO:0000269\|PubMed:19919825}.


Subunit:		Interacts with HIF3A isoform 2 (via C-terminus domain) (PubMed:21546903). Interacts with BAD, BOK, BIK, BAX, BAK1, and TPT1. Interacts with BBC3, BMF and PMAIP1 (PubMed:15550399, PubMed:18589438). Interacts with BOP. Interacts with BCL2L11; this interaction may sequester BCL2L11 and prevent its pro-apoptotic activity (PubMed:16543145, PubMed:27013495, PubMed:15550399). Interacts with GIMAP5 and HSPA8/HSC70; the interaction between HSPA8 and MCL1 is impaired in the absence of GIMAP5 (PubMed:21502331). {ECO:0000269\|PubMed:15550399, ECO:0000269\|PubMed:16543145, ECO:0000269\|PubMed:18589438, ECO:0000269\|PubMed:21502331, ECO:0000269\|PubMed:21546903, ECO:0000269\|PubMed:27013495}.
Subcellular location:		Membrane {ECO:0000250}; Single-pass membrane protein {ECO:0000250}. Cytoplasm {ECO:0000250}. Mitochondrion. Nucleus, nucleoplasm {ECO:0000250}. Note=Cytoplasmic, associated with mitochondria.
Induction:		Up-regulated by IL3 and CSF2. Up-regulated in murine embryonal carcinoma cells in response to retinoic acid treatment. Levels reach a maximum after 4 hours, are decreased after 8 hours and are back to maximum after 12 hours. Levels are decreased after 24 hours and back to basal levels after 48 hours. Expression remains constant in retinoic acid-treated embryonic stem cells. {ECO:0000269\|PubMed:9655929, ECO:0000269\|PubMed:9671497}.
Ptm:		Cleaved by CASP3 during apoptosis, yielding a pro-apoptotic C- terminal fragment. {ECO:0000250}.
Ptm:		Rapidly degraded in the absence of phosphorylation in the PEST region. {ECO:0000250}.
Ptm:		Phosphorylated on Ser-140, by GSK3, in response to IL3/interleukin-3 withdrawal. Phosphorylation at Ser-140 induces ubiquitination and proteasomal degradation, abrogating the anti- apoptotic activity. Treatment with taxol or okadaic acid induces phosphorylation on additional sites. {ECO:0000269\|PubMed:16543145}.
Ptm:		Ubiquitinated. Ubiquitination is induced by phosphorylation at Ser-140 (By similarity). {ECO:0000250}.
Miscellaneous:		[Isoform 2]: This isoform is more stable than isoform 1 in cells undergoing apoptosis. {ECO:0000305}.
Similarity:		Belongs to the Bcl-2 family. {ECO:0000305}.