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PDBsum entry 4b65
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Oxidoreductase
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PDB id
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4b65
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References listed in PDB file
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Key reference
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Title
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Structural insight into the mechanism of oxygen activation and substrate selectivity of flavin-Dependent n-Hydroxylating monooxygenases.
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Authors
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S.Franceschini,
M.Fedkenheuer,
N.J.Vogelaar,
H.H.Robinson,
P.Sobrado,
A.Mattevi.
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Ref.
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Biochemistry, 2012,
51,
7043-7045.
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PubMed id
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Abstract
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SidA from the human pathogen Aspergillus fumigatus catalyzes the generation of
N(5)-hydroxyornithine in the biosynthesis of siderophores, a reaction essential
for virulence. The crystal structures of SidA in complex with ornithine and
lysine reveal the geometry of the interactions among flavin, NADP(+), and the
substrate amine group that underlie the hydroxylation reaction. The structural
elucidation of the enzyme in complex with arginine provides insight into the
role of electrostatics and hydrogen bonding in the mechanism of oxygen
activation in this family of enzymes.
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