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PDBsum entry 4ad8
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DNA binding protein
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PDB id
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4ad8
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References listed in PDB file
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Key reference
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Title
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Structural and functional characterization of an smc-Like protein recn: new insights into double-Strand break repair.
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Authors
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S.Pellegrino,
J.Radzimanowski,
D.De sanctis,
E.Boeri erba,
S.Mcsweeney,
J.Timmins.
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Ref.
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Structure, 2012,
20,
2076-2089.
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PubMed id
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Abstract
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Repair of DNA double-strand breaks (DSBs) is essential for cell survival and
maintaining genome integrity. DSBs are repaired in a stepwise manner by
homologous recombination. Here, we focused on the early steps of DSB repair,
including DSB recognition, which is still only poorly understood. In
prokaryotes, this process has been proposed to involve the RecN protein, a
member of the structural maintenance of chromosome (SMC) protein family, which
include key eukaryotic and prokaryotic proteins such as cohesin, condensin, and
Rad50. An extensive high- and low-resolution structural analysis of Deinococcus
radiodurans RecN using a combination of protein crystallography and small-angle
X-ray scattering enabled us to assemble a quasi-atomic model of the entire RecN
protein, representing the complete structure of a SMC-like protein. These
results, together with a thorough biochemical and mutational study of RecN,
allow us to propose a model for the role of RecN in DSB repair.
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