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UniProt functional annotation for Q837U7 | ||
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| UniProt code: Q837U7. |
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| Organism: | |
Enterococcus faecalis (strain ATCC 700802 / V583). |
| Taxonomy: | |
Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae; Enterococcus. |
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| Function: | |
Catalyzes the phosphorolysis of N-carbamoylputrescine to form carbamoyl phosphate and putrescine. Is involved in the degradation pathway of the polyamine agmatine. Also has weak activity with ornithine and cadaverine. {ECO:0000269|PubMed:22363663, ECO:0000269|PubMed:4621632}. |
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| Catalytic activity: | |
Reaction=carbamoyl phosphate + putrescine = H(+) + N- carbamoylputrescine + phosphate; Xref=Rhea:RHEA:21936, ChEBI:CHEBI:15378, ChEBI:CHEBI:43474, ChEBI:CHEBI:58228, ChEBI:CHEBI:58318, ChEBI:CHEBI:326268; EC=2.1.3.6; Evidence={ECO:0000269|PubMed:17028272, ECO:0000269|PubMed:22363663, ECO:0000269|PubMed:4621632}; PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:21938; Evidence={ECO:0000269|PubMed:17028272, ECO:0000269|PubMed:22363663, ECO:0000269|PubMed:4621632}; |
| Activity regulation: | |
Inhibited by spermidine (PubMed:116850). Inhibited by N-(phosphonoacetyl)-putrescine (PubMed:17028272, PubMed:4621632, PubMed:22363663). Inhibited by N-(phosphonoacetyl)-L-ornithine (PubMed:22363663). {ECO:0000269|PubMed:116850, ECO:0000269|PubMed:17028272, ECO:0000269|PubMed:22363663, ECO:0000269|PubMed:4621632}. |
| Biophysicochemical properties: | |
Kinetic parameters: KM=58 uM for carbamoyl phosphate {ECO:0000269|PubMed:17028272}; KM=2.3 mM for putrescine {ECO:0000269|PubMed:17028272}; KM=1.41 mM for putrescine {ECO:0000269|PubMed:22363663}; KM=36.4 mM for ornithine {ECO:0000269|PubMed:22363663}; pH dependence: Optimum pH is 6.7 with 10 mM putrescine and 10 mM carbamoyl phosphate, 7.8 with 0.2 mM putrescine and 0.2 mM carbamoyl phosphate, and 9.0 with 0.5 mM putrescine and 10 mM carbamoyl phosphate. {ECO:0000269|PubMed:116850}; |
| Pathway: | |
Amine and polyamine biosynthesis; putrescine biosynthesis via agmatine pathway; putrescine from N-carbamoylputrescine (transferase route): step 1/1. |
| Subunit: | |
Homotrimer. {ECO:0000269|PubMed:116850, ECO:0000269|PubMed:17028272}. |
| Subcellular location: | |
Cytoplasm {ECO:0000250}. |
| Similarity: | |
Belongs to the aspartate/ornithine carbamoyltransferase superfamily. PTCase family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.