PDBsum entry 4nzh

Oxidoreductase

PDB id: 4nzh

Contents

Protein chain

448 a.a.

Ligands

FAD

ORN

GOL ×4

Waters ×204

PDB id:

4nzh

Name:

Oxidoreductase

Title:

A. Fumigatus flavin-dependent ornithine monooxygenase r279a mutant

Structure:

L-ornithine n5 monooxygenase. Chain: a. Synonym: l-ornithine n5-oxygenase. Engineered: yes. Mutation: yes

Source:

Aspergillus fumigatus. Organism_taxid: 746128. Gene: sida. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

2.00Å R-factor: 0.208 R-free: 0.243

Authors:

R.Robinson,S.Franceschini,P.Sobrado

Key ref:

R.Robinson et al. A. Fumigatus flavin-Dependent ornithine monooxygenase mutant. To be published, .

Date:

12-Dec-13 Release date: 04-Mar-15

Protein chain

E9QYP0  (SIDA_ASPFU) -  L-ornithine N(5)-monooxygenase from Aspergillus fumigatus (strain ATCC MYA-4609 / CBS 101355 / FGSC A1100 / Af293)

Seq: 501 a.a.

Struc: 448 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.1.14.13.196 - L-ornithine N(5)-monooxygenase [NAD(P)H].
• Reaction:
  1. L-ornithine + NADPH + O2 = N₅-hydroxy-L-ornithine + NADP⁺ + H2O
  2. L-ornithine + NADH + O2 = N₅-hydroxy-L-ornithine + NAD⁺ + H2O

L-ornithine (Bound ligand (Het Group name = ORN) corresponds exactly) + NADPH + O2 = N(5)-hydroxy-L-ornithine + NADP(+) + H2O

L-ornithine (Bound ligand (Het Group name = ORN) corresponds exactly) + NADH + O2 = N(5)-hydroxy-L-ornithine + NAD(+) + H2O

• Cofactor: FAD

FAD (Bound ligand (Het Group name = FAD) corresponds exactly)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site