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PDBsum entry 3wmv
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Sugar binding protein
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PDB id
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3wmv
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References listed in PDB file
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Key reference
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Title
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Crystal structure of mytilec, A galactose-Binding lectin from the mussel mytilus galloprovincialis with cytotoxicity against certain cancer cell types.
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Authors
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D.Terada,
F.Kawai,
H.Noguchi,
S.Unzai,
I.Hasan,
Y.Fujii,
S.Y.Park,
Y.Ozeki,
J.R.Tame.
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Ref.
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Sci Rep, 2016,
6,
28344.
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PubMed id
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Abstract
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MytiLec is a lectin, isolated from bivalves, with cytotoxic activity against
cancer cell lines that express globotriaosyl ceramide,
Galα(1,4)Galβ(1,4)Glcα1-Cer, on the cell surface. Functional analysis shows
that the protein binds to the disaccharide melibiose, Galα(1,6)Glc, and the
trisaccharide globotriose, Galα(1,4)Galβ(1,4)Glc. Recombinant MytiLec
expressed in bacteria showed the same haemagglutinating and cytotoxic activity
against Burkitt's lymphoma (Raji) cells as the native form. The crystal
structure has been determined to atomic resolution, in the presence and absence
of ligands, showing the protein to be a member of the β-trefoil family, but
with a mode of ligand binding unique to a small group of related trefoil
lectins. Each of the three pseudo-equivalent binding sites within the monomer
shows ligand binding, and the protein forms a tight dimer in solution. An
engineered monomer mutant lost all cytotoxic activity against Raji cells, but
retained some haemagglutination activity, showing that the quaternary structure
of the protein is important for its cellular effects.
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