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UniProt functional annotation for O26314 | ||
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| UniProt code: O26314. |
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| Organism: | |
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum). |
| Taxonomy: | |
Archaea; Euryarchaeota; Methanomada group; Methanobacteria; Methanobacteriales; Methanobacteriaceae; Methanothermobacter. |
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| Function: | |
Involved in DNA uracil repair (PubMed:17012282, PubMed:19240141, PubMed:20129830). Recognizes DNA uracil residues within double-stranded DNA and initiates DNA-U repair by endonucleotic incision on the 5'-side of the 2'-d-uridine residue, irrespective of the nature of the opposing nucleotide (PubMed:17012282, PubMed:19240141, PubMed:20129830). In addition, acts as an apurinic/apyrimidinic (AP) endonuclease hydrolyzing the DNA phosphodiester backbone immediately at the 5'-side of AP sites, and as a 3'-5' exonuclease (PubMed:15725624, PubMed:17012282). Strongly binds to double-stranded DNA (PubMed:15725624). {ECO:0000269|PubMed:15725624, ECO:0000269|PubMed:17012282, ECO:0000269|PubMed:19240141, ECO:0000269|PubMed:20129830}. |
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| Catalytic activity: | |
Reaction=(DNA)-(deoxyribonucleoside 5'-phosphate)-(deoxyribose 5'- phosphate)-(deoxyribonucleoside 5'-phosphate) = (DNA)-3'- (deoxyribonucleoside 5'-phosphate)-(2,3-dehydro-2,3-deoxyribose 5'- phosphate) + H(+) + phospho-5'-(DNA); Xref=Rhea:RHEA:66592, Rhea:RHEA-COMP:13180, Rhea:RHEA-COMP:16897, Rhea:RHEA-COMP:17067, ChEBI:CHEBI:15378, ChEBI:CHEBI:136412, ChEBI:CHEBI:157695, ChEBI:CHEBI:167181; EC=4.2.99.18; Evidence={ECO:0000269|PubMed:15725624, ECO:0000269|PubMed:17012282}; |
| Catalytic activity: | |
Reaction=Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.; EC=3.1.11.2; Evidence={ECO:0000269|PubMed:15725624, ECO:0000269|PubMed:17012282}; |
| Cofactor: | |
Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000269|PubMed:15725624, ECO:0000269|PubMed:17012282}; Note=Can also use Mn(2+), Co(2+) and Ni(2+). {ECO:0000269|PubMed:15725624}; |
| Disruption phenotype: | |
Depletion leads to the loss of DNA-U repair. {ECO:0000269|PubMed:19240141}. |
| Miscellaneous: | |
The insertion of the side chain of Arg-209 into the DNA helical base stack seems to be crucial for the uridine recognition. {ECO:0000269|PubMed:20434457}. |
| Similarity: | |
Belongs to the DNA repair enzymes AP/exoA family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.