PDBsum entry 3vwm

Hydrolase

PDB id: 3vwm

Contents

Protein chain

384 a.a.

Ligands

GOL ×5

MES ×3

SO4 ×2

Waters ×425

PDB id:

3vwm

Name:

Hydrolase

Title:

Crystal structure of 6-aminohexanoate-dimer hydrolase g181d/r187a/h266n/d370y mutant

Structure:

6-aminohexanoate-dimer hydrolase. Chain: a. Synonym: nylon oligomers-degrading enzyme eii, nylon oligomers- degrading enzyme eii'. Engineered: yes. Mutation: yes. Other_details: the fusion protein of residues 1-21 from nylon oligomers-degrading enzyme eii (unp p07061) and residues 22-392 from nylon oligomers-degrading enzyme eii' (unp q59710)

Source:

Flavobacterium. Organism_taxid: 261, 408956. Strain: k172, ki723t1. Gene: nylb, nylb'. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

1.60Å R-factor: 0.193 R-free: 0.218

Authors:

Y.Kawashima,N.Shibata,S.Negoro,Y.Higuchi

Key ref:

S.Negoro et al. Structural, Kinetic and theoretical analyses of hydro mutants altering in the directionality and equilibriu of reversible amide-Synthetic/hydrolytic reaction. To be published, .

Date:

30-Aug-12 Release date: 16-Oct-13

Protein chain

P07061  (NYLB_PAEUR) -  6-aminohexanoate-dimer hydrolase from Paenarthrobacter ureafaciens

Seq: 392 a.a.

Struc: 384 a.a.*

Protein chain

P07062  (NYLB2_PAEUR) -  6-aminohexanoate-dimer hydrolase from Paenarthrobacter ureafaciens

Seq: 392 a.a.

Struc: 384 a.a.*

* PDB and UniProt seqs differ at 46 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.3.5.1.46 - 6-aminohexanoate-oligomer exohydrolase.
• Reaction:
  1. [N-(6-aminohexanoyl)](n) + H2O = [N-(6-aminohexanoyl)](n-1) + 6-aminohexanoate
  2. N-(6-aminohexanoyl)-6-aminohexanoate + H2O = 2 6-aminohexanoate

Molecule diagrams generated from .mol files obtained from the KEGG ftp site