PDBsum entry 3voo

Oxidoreductase

PDB id: 3voo

Contents

Protein chain

407 a.a.

Ligands

HEM

Waters ×60

PDB id:

3voo

Name:

Oxidoreductase

Title:

Cytochrome p450sp alpha (cyp152b1) mutant a245e

Structure:

Fatty acid alpha-hydroxylase. Chain: a. Fragment: residues 9-415. Engineered: yes. Mutation: yes

Source:

Sphingomonas paucimobilis. Organism_taxid: 13689. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

2.34Å R-factor: 0.198 R-free: 0.234

Authors:

T.Fujishiro,O.Shoji,H.Sugimoto,Y.Shiro,Y.Watanabe

Key ref:

T.Fujishiro et al. Crystal structure of hydrogen peroxide-Dependent cyto p450sp alpha mutants. To be published, .

Date:

31-Jan-12 Release date: 06-Feb-13

Protein chain

O24782  (O24782_SPHPI) -  Cytochrome P450 from Sphingomonas paucimobilis

Seq: 415 a.a.

Struc: 407 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.1.11.2.4 - fatty-acid peroxygenase.
• Reaction:
  1. a 1,2-saturated fatty acid + H2O2 = a 2-hydroxy fatty acid + H2O
  2. a 2,3-saturated fatty acid + H2O2 = a 3-hydroxy fatty acid + H2O
• Cofactor: Heme-thiolate

Molecule diagrams generated from .mol files obtained from the KEGG ftp site