PDBsum entry 3vmm

Ligase

PDB id: 3vmm

Contents

Protein chain

471 a.a.

Ligands

ADP

P0D

Metals

_MG ×2

Waters ×147

PDB id:

3vmm

Name:

Ligase

Title:

Crystal structure of bacd, an l-amino acid dipeptide ligase from bacillus subtilis

Structure:

Alanine-anticapsin ligase bacd. Chain: a. Synonym: bacilysin synthetase, l-amino acid ligase. Engineered: yes

Source:

Bacillus subtilis. Organism_taxid: 1423. Gene: bacd, bsu37710, ipa-83d. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

2.50Å R-factor: 0.194 R-free: 0.229

Authors:

Y.Shomura,Y.Higuchi

Key ref:

Y.Shomura et al. (2012). Structural and enzymatic characterization of BacD, an L-amino acid dipeptide ligase from Bacillus subtilis. Protein Sci, 21, 707-716. PubMed id: 22407814

Date:

14-Dec-11 Release date: 11-Apr-12

Protein chain

P39641  (BACD_BACSU) -  Alanine--anticapsin ligase from Bacillus subtilis (strain 168)

Seq: 472 a.a.

Struc: 471 a.a.

Enzyme reactions

• Enzyme class: E.C.6.3.2.49 - L-alanine--L-anticapsin ligase.
• Reaction: L-anticapsin + L-alanine + ATP = bacilysin + ADP + phosphate + H⁺

L-anticapsin + L-alanine + ATP = bacilysin (Bound ligand (Het Group name = ADP) corresponds exactly) + ADP + phosphate + H(+)

• Cofactor: Mn(2+) or Mg(2+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

Protein Sci 21:707-716 (2012)

PubMed id: 22407814

Structural and enzymatic characterization of BacD, an L-amino acid dipeptide ligase from Bacillus subtilis.

Y.Shomura, E.Hinokuchi, H.Ikeda, A.Senoo, Y.Takahashi, J.Saito, H.Komori, N.Shibata, Y.Yonetani, Y.Higuchi.

ABSTRACT

No abstract given.