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UniProt functional annotation for P9WPA5 | ||
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| UniProt code: P9WPA5. |
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| Organism: | |
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv). |
| Taxonomy: | |
Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. |
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| Function: | |
Reversibly transfers an adenylyl group from ATP to 4'- phosphopantetheine, yielding dephospho-CoA (dPCoA) and pyrophosphate. {ECO:0000255|HAMAP-Rule:MF_00151, ECO:0000269|PubMed:20851704, ECO:0000269|Ref.5}. |
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| Catalytic activity: | |
Reaction=ATP + D-pantetheine 4'-phosphate + H(+) = 3'-dephospho-CoA + diphosphate; Xref=Rhea:RHEA:19801, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57328, ChEBI:CHEBI:61723; EC=2.7.7.3; Evidence={ECO:0000255|HAMAP- Rule:MF_00151, ECO:0000269|PubMed:20851704, ECO:0000269|Ref.5}; |
| Cofactor: | |
Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000255|HAMAP-Rule:MF_00151}; |
| Activity regulation: | |
Regulated by Coenzyme A (CoA) through feedback inhibition. {ECO:0000305|PubMed:21543857, ECO:0000305|Ref.5}. |
| Biophysicochemical properties: | |
Kinetic parameters: KM=6.7 uM for 3'-dephospho-CoA {ECO:0000269|PubMed:20851704}; Note=kcat is 288.5 min(-1) for the reverse reaction, with diphosphate and 3'-dephospho-CoA as substrates, which is nearly identical to that of the forward reaction fitted to the Hill equation. The enzyme seems to utilize a nonrapid-equilibrium random bi-bi mechanism with a preferred pathway to the ternary complex. A sigmoidal response is observed when ATP was varied at different fixed concentrations of pantetheine 4'-phosphate. Also exhibits nonhyperbolic kinetics when pantetheine 4'-phosphate was varied at a fixed saturating concentration of ATP. {ECO:0000269|PubMed:20851704}; |
| Pathway: | |
Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)- pantothenate: step 4/5. {ECO:0000255|HAMAP-Rule:MF_00151}. |
| Subunit: | |
Homohexamer; dimer of trimers. {ECO:0000255|HAMAP- Rule:MF_00151, ECO:0000269|Ref.5, ECO:0000305|PubMed:15322293}. |
| Subcellular location: | |
Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00151}. |
| Similarity: | |
Belongs to the bacterial CoaD family. {ECO:0000255|HAMAP- Rule:MF_00151}. |
Annotations taken from UniProtKB at the EBI.