PDBsum entry 3u3r

Transferase

PDB id: 3u3r

Contents

Protein chain

288 a.a.

Ligands

NPO ×2

A3P

Waters ×100

PDB id:

3u3r

Name:

Transferase

Title:

Crystal structure of d249g mutated human sult1a1 bound to pap and p- nitrophenol

Structure:

Sulfotransferase 1a1. Chain: a. Fragment: unp residues 1-295. Synonym: st1a1, aryl sulfotransferase 1, hast1/hast2, phenol sulfotransferase 1, phenol-sulfating phenol sulfotransferase 1, p-pst 1, st1a3, thermostable phenol sulfotransferase, ts-pst. Engineered: yes. Mutation: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Strain: homo sapiens. Gene: hsult1a1 d249g mutation, ok/sw-cl.88, stp, stp1, sult1a1. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

2.36Å R-factor: 0.170 R-free: 0.217

Authors:

C.Guttman,I.Berger,A.Aharoni,R.Zarivach

Key ref:

I.Berger et al. (2011). The molecular basis for the broad substrate specificity of human sulfotransferase 1A1. Plos One, 6, e26794. PubMed id: 22069470

Date:

06-Oct-11 Release date: 16-Nov-11

Protein chain

P50225  (ST1A1_HUMAN) -  Sulfotransferase 1A1 from Homo sapiens

Seq: 295 a.a.

Struc: 288 a.a.*

* PDB and UniProt seqs differ at 3 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.2.8.2.1 - aryl sulfotransferase.
• Reaction: a phenol + 3'-phosphoadenylyl sulfate = an aryl sulfate + adenosine 3',5'-bisphosphate + H⁺

phenol + 3'-phosphoadenylyl sulfate (Bound ligand (Het Group name = NPO) matches with 70.00% similarity) = aryl sulfate (Bound ligand (Het Group name = A3P) corresponds exactly) + adenosine 3',5'-bisphosphate + H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

Plos One 6:e26794 (2011)

PubMed id: 22069470

The molecular basis for the broad substrate specificity of human sulfotransferase 1A1.

I.Berger, C.Guttman, D.Amar, R.Zarivach, A.Aharoni.

ABSTRACT

No abstract given.