PDBsum entry 3swi

Transferase

PDB id: 3swi

Contents

Protein chain

419 a.a.

Ligands

MOE ×2

PG0 ×3

UD2

UPN

Metals

_MG

Waters ×113

PDB id:

3swi

Name:

Transferase

Title:

E. Cloacae mura in complex with enolpyruvyl-udp-n-acetylgalactosamine and covalent adduct of pep with cys115

Structure:

Udp-n-acetylglucosamine 1-carboxyvinyltransferase. Chain: a. Synonym: enoylpyruvate transferase, udp-n-acetylglucosamine enolpyruvyl transferase, ept. Engineered: yes. Mutation: yes

Source:

Enterobacter cloacae. Organism_taxid: 716541. Strain: atcc 13047 / dsm 30054 / nbrc 13535 / ncdc 279-56. Gene: mura, murz, ecl_04571. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

2.80Å R-factor: 0.188 R-free: 0.263

Authors:

J.-Y.Zhu,Y.Yang,E.Schonbrunn

Key ref:

J.Y.Zhu et al. (2012). Functional consequence of covalent reaction of phosphoenolpyruvate with UDP-N-acetylglucosamine 1-carboxyvinyltransferase (MurA). J Biol Chem, 287, 12657-12667. PubMed id: 22378791

Date:

13-Jul-11 Release date: 07-Mar-12

Protein chain

P33038  (MURA_ENTCC) -  UDP-N-acetylglucosamine 1-carboxyvinyltransferase from Enterobacter cloacae subsp. cloacae (strain ATCC 13047 / DSM 30054 / NBRC 13535 / NCTC 10005 / WDCM 00083 / NCDC 279-56)

Seq: 419 a.a.

Struc: 419 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.2.5.1.7 - UDP-N-acetylglucosamine 1-carboxyvinyltransferase.
• Pathway: Peptidoglycan Biosynthesis (Part 1)
• Reaction: phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine = UDP-N-acetyl-3- O-(1-carboxyvinyl)-alpha-D-glucosamine + phosphate

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Added reference

J Biol Chem 287:12657-12667 (2012)

PubMed id: 22378791

Functional consequence of covalent reaction of phosphoenolpyruvate with UDP-N-acetylglucosamine 1-carboxyvinyltransferase (MurA).

J.Y.Zhu, Y.Yang, H.Han, S.Betzi, S.H.Olesen, F.Marsilio, E.Schönbrunn.

ABSTRACT

No abstract given.