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UniProt functional annotation for P56221 | ||
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| UniProt code: P56221. |
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| Organism: | |
Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast fungus) (Pyricularia oryzae). |
| Taxonomy: | |
Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes; Sordariomycetidae; Magnaporthales; Pyriculariaceae; Pyricularia. |
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| Function: | |
Scytalone dehydratase; part of the gene cluster that mediates the biosynthesis of dihydroxynaphthalene melanin, a bluish-green pigment and a structural component of the conidial wall (PubMed:9571787). Within the pathway, catalyzes the dehydration of scytalone as well as of vermelone (PubMed:9571787, PubMed:9466791, PubMed:9466792, PubMed:9539706, PubMed:10320327, PubMed:10386945, PubMed:10386946, PubMed:10694394, PubMed:10913266, PubMed:10636235, PubMed:11790103, PubMed:12413868, PubMed:15056895). Is also able to dehydrate the alternate substrate 2,3-dihydro-2,5-dihydroxy-4H- benzopyran-4-one (DDBO) to 5-hydroxy-4H-1-benzopyran-4-one (HBO) (PubMed:9466791, PubMed:9466792, PubMed:10320327, PubMed:10386945, PubMed:11790103). {ECO:0000269|PubMed:10320327, ECO:0000269|PubMed:10386945, ECO:0000269|PubMed:10386946, ECO:0000269|PubMed:10636235, ECO:0000269|PubMed:10694394, ECO:0000269|PubMed:10913266, ECO:0000269|PubMed:11790103, ECO:0000269|PubMed:12413868, ECO:0000269|PubMed:15056895, ECO:0000269|PubMed:9466791, ECO:0000269|PubMed:9466792, ECO:0000269|PubMed:9539706, ECO:0000269|PubMed:9571787}. |
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| Catalytic activity: | |
Reaction=scytalone = 1,3,8-trihydroxynaphthalene + H2O; Xref=Rhea:RHEA:24396, ChEBI:CHEBI:15377, ChEBI:CHEBI:16945, ChEBI:CHEBI:18393; EC=4.2.1.94; Evidence={ECO:0000269|PubMed:10320327, ECO:0000269|PubMed:10386945, ECO:0000269|PubMed:10386946, ECO:0000269|PubMed:10636235, ECO:0000269|PubMed:10694394, ECO:0000269|PubMed:10913266, ECO:0000269|PubMed:11790103, ECO:0000269|PubMed:12413868, ECO:0000269|PubMed:15056895, ECO:0000269|PubMed:9466791, ECO:0000269|PubMed:9466792, ECO:0000269|PubMed:9539706, ECO:0000269|PubMed:9571787}; |
| Activity regulation: | |
(N-phenoxypropyl)-carboxamides such as carpropamid and derivatives of norephedrine act as inhibitors of scytalone dehydratase activity. {ECO:0000269|PubMed:10386945, ECO:0000269|PubMed:10386946, ECO:0000269|PubMed:10882002, ECO:0000269|PubMed:12413868, ECO:0000269|PubMed:15056895, ECO:0000269|PubMed:15382507}. |
| Biophysicochemical properties: | |
Kinetic parameters: KM=15 uM for 2,3-dihydro-2,5-dihydroxy-4H-benzopyran-4-one (DDBO) {ECO:0000269|PubMed:10320327, ECO:0000269|PubMed:9466791}; KM=33 uM for scytalone {ECO:0000269|PubMed:10320327}; KM=31 uM for vermelone {ECO:0000269|PubMed:10320327, ECO:0000269|PubMed:11790103}; |
| Pathway: | |
Pigment biosynthesis; melanin biosynthesis. {ECO:0000269|PubMed:9571787}. |
| Subunit: | |
Homotrimer (PubMed:7866745). Each subunit contains an active site, located in the central part of the hydrophobic core of the monomer, which functions independently (PubMed:7866745). {ECO:0000269|PubMed:7866745}. |
| Subcellular location: | |
Endosome {ECO:0000250|UniProtKB:O14434}. |
| Induction: | |
Expression is induced in the stationary phase, when melanin synthesis occurs (PubMed:9571787). Expression is specifically induced during appressorium formation (PubMed:15378734). {ECO:0000269|PubMed:15378734, ECO:0000269|PubMed:9571787}. |
| Similarity: | |
Belongs to the scytalone dehydratase family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.