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UniProt functional annotation for O74859 | ||
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| UniProt code: O74859. |
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| Organism: | |
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast). |
| Taxonomy: | |
Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina; Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces. |
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| Function: | |
DNA-binding protein involved in single-strand DNA break repair, double-strand DNA break repair and base excision repair. Resolves abortive DNA ligation intermediates formed either at base excision sites, or when DNA ligases attempt to repair non-ligatable breaks induced by reactive oxygen species (PubMed:24362567, PubMed:21984208). Catalyzes the release of adenylate groups covalently linked to 5'-phosphate termini, resulting in the production of 5'- phosphate termini that can be efficiently rejoined (PubMed:24362567, PubMed:21984210). Likewise, catalyzes the release of 3'-linked guanosine (DNAppG) and inosine (DNAppI) from DNA, but has higher specific activity with 5'-linked adenosine (AppDNA) (PubMed:26007660). {ECO:0000269|PubMed:21984210, ECO:0000269|PubMed:24362567, ECO:0000269|PubMed:26007660, ECO:0000305, ECO:0000305|PubMed:21984208}. |
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| Catalytic activity: | |
Reaction=adenosine-5'-diphospho-5'-(DNA) + H2O = AMP + 2 H(+) + phospho-5'-(DNA); Xref=Rhea:RHEA:52128, Rhea:RHEA-COMP:13180, Rhea:RHEA-COMP:13181, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:136412, ChEBI:CHEBI:136413, ChEBI:CHEBI:456215; EC=3.6.1.71; Evidence={ECO:0000269|PubMed:21984210, ECO:0000269|PubMed:24362567}; |
| Catalytic activity: | |
Reaction=adenosine-5'-diphospho-5'-(ribonucleotide)-(DNA) + H2O = 5'- phospho-(ribonucleotide)-(DNA) + AMP + 2 H(+); Xref=Rhea:RHEA:52132, Rhea:RHEA-COMP:13182, Rhea:RHEA-COMP:13183, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:136414, ChEBI:CHEBI:136415, ChEBI:CHEBI:456215; EC=3.6.1.71; Evidence={ECO:0000269|PubMed:21984210}; |
| Catalytic activity: | |
Reaction=(DNA)-3'-diphospho-5'-guanosine + H2O = (DNA)-3'-phosphate + GMP + 2 H(+); Xref=Rhea:RHEA:52140, Rhea:RHEA-COMP:13186, Rhea:RHEA- COMP:13187, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:58115, ChEBI:CHEBI:136419, ChEBI:CHEBI:136420; EC=3.6.1.72; Evidence={ECO:0000269|PubMed:26007660}; |
| Subunit: | |
Monomer. {ECO:0000269|PubMed:21984208, ECO:0000269|PubMed:21984210}. |
| Subcellular location: | |
Nucleus {ECO:0000269|PubMed:16823372}. Cytoplasm {ECO:0000269|PubMed:16823372}. |
Annotations taken from UniProtKB at the EBI.