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UniProt functional annotation for O81086 | ||
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| UniProt code: O81086. |
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| Organism: | |
Abies grandis (Grand fir) (Pinus grandis). |
| Taxonomy: | |
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Pinopsida; Pinidae; Pinales; Pinaceae; Abies. |
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| Function: | |
Converts farnesyl diphosphate to alpha-bisabolene (PubMed:9618485, PubMed:9539701, PubMed:22153510). Involved in defensive oleoresin formation in conifers in response to insect attack or other injury (PubMed:9618485, PubMed:9539701). Involved in sesquiterpene (C15) olefins biosynthesis (PubMed:9618485, PubMed:9539701). {ECO:0000269|PubMed:22153510, ECO:0000269|PubMed:9539701, ECO:0000269|PubMed:9618485}. |
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| Catalytic activity: | |
Reaction=(2E,6E)-farnesyl diphosphate = (E,R)-alpha-bisabolene + diphosphate; Xref=Rhea:RHEA:25436, ChEBI:CHEBI:33019, ChEBI:CHEBI:49243, ChEBI:CHEBI:175763; EC=4.2.3.38; Evidence={ECO:0000269|PubMed:22153510, ECO:0000269|PubMed:9618485}; |
| Cofactor: | |
Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000269|PubMed:22153510, ECO:0000269|PubMed:9618485}; Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000269|PubMed:9618485}; Note=Binds 3 divalent metal cations per subunit (PubMed:22153510). Can use either Mg(2+) or Mn(2+) (PubMed:9618485). {ECO:0000269|PubMed:22153510, ECO:0000269|PubMed:9618485}; |
| Cofactor: | |
Name=K(+); Xref=ChEBI:CHEBI:29103; Evidence={ECO:0000250}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=49.5 uM for (2E,6E)-farnesyl diphosphate {ECO:0000269|PubMed:22153510}; |
| Pathway: | |
Terpene metabolism; oleoresin biosynthesis. {ECO:0000305}. |
| Subcellular location: | |
Cytoplasm {ECO:0000305}. |
| Induction: | |
By wounding. {ECO:0000269|PubMed:9618485}. |
| Domain: | |
The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity, presumably through binding to Mg(2+). |
| Miscellaneous: | |
The conserved 25-Arg-Arg-26 motif may play a role in the isomerization step of the terpenoid cyclization reaction sequence. |
| Similarity: | |
Belongs to the terpene synthase family. Tpsd subfamily. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.