PDBsum entry 3ryb

Peptide binding protein

PDB id: 3ryb

Contents

Protein chain

563 a.a.

Ligands

SER-LEU-SER-GLN-SER-LEU-SER-GLN-SER

Waters ×859

PDB id:

3ryb

Name:

Peptide binding protein

Title:

Lactococcal oppa complexed with slsqslsqs

Structure:

Oligopeptide-binding protein oppa. Chain: a. Engineered: yes. Oligopeptide. Chain: b. Engineered: yes

Source:

Lactococcus lactis. Organism_taxid: 416870. Strain: mg1363. Gene: llmg_0701, oppa. Expressed in: lactococcus lactis. Expression_system_taxid: 416870. Synthetic: yes

Resolution:

1.50Å R-factor: 0.165 R-free: 0.201

Authors:

R.P.-A.Berntsson,A.-M.W.H.Thunnissen,B.Poolman,D.-J.Slotboom

Key ref:

R.P.Berntsson et al. (2011). Importance of a hydrophobic pocket for peptide binding in lactococcal OppA. J Bacteriol, 193, 4254-4256. PubMed id: 21665971

Date:

11-May-11 Release date: 29-Jun-11

Protein chain

A2RJ53  (A2RJ53_LACLM) -  Oligopeptide-binding protein oppA from Lactococcus lactis subsp. cremoris (strain MG1363)

Seq: 600 a.a.

Struc: 563 a.a.

J Bacteriol 193:4254-4256 (2011)

PubMed id: 21665971

Importance of a hydrophobic pocket for peptide binding in lactococcal OppA.

R.P.Berntsson, A.M.Thunnissen, B.Poolman, D.J.Slotboom.

ABSTRACT

Lactococcal oligopeptide-binding protein A (OppA) binds peptides with widely varied lengths and sequences. We previously hypothesized that a hydrophobic pocket in OppA preferentially binds a hydrophobic peptide side chain and thus determines its binding register. Two crystal structures of OppA with different nonapeptides now indeed show binding in different registers.