UniProt functional annotation for Q7BJX9

UniProt code: Q7BJX9.

Organism:		Plesiomonas shigelloides (Aeromonas shigelloides).
Taxonomy:		Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacterales incertae sedis; Plesiomonas.
Function:		Catalyzes the epimerization of UDP-N-acetylglucosamine (UDP- GlcNAc) to UDP-N-acetylgalactosamine (UDP-GalNAc). Has very low epimerase activity with UDP-Glc and UDP-Gal. Plays a role in the biosynthesis of 2-acetamino-2-deoxy-L-altruronic acid, a building block of the O-antigen in bacterial lipopolysaccharide (LPS). {ECO:0000269|PubMed:12484781, ECO:0000269|PubMed:21810411, ECO:0000305}.
Catalytic activity:		Reaction=UDP-N-acetyl-alpha-D-glucosamine = UDP-N-acetyl-alpha-D- galactosamine; Xref=Rhea:RHEA:20517, ChEBI:CHEBI:57705, ChEBI:CHEBI:67138; EC=5.1.3.7; Evidence={ECO:0000269|PubMed:12484781, ECO:0000269|PubMed:21810411};
Cofactor:		Name=NAD(+); Xref=ChEBI:CHEBI:57540; Evidence={ECO:0000269|PubMed:12484781, ECO:0000269|PubMed:21384454, ECO:0000269|PubMed:21810411}; Note=Binds 1 NAD(+) per subunit. {ECO:0000269|PubMed:12484781, ECO:0000269|PubMed:21384454, ECO:0000269|PubMed:21810411};
Biophysicochemical properties:		Kinetic parameters: KM=131 uM for UDP-GalNAc {ECO:0000269|PubMed:12484781}; KM=137 uM for UDP-GalNAc {ECO:0000269|PubMed:12484781}; Note=kcat is 461 (s-1) with UDP-GlcNAc. kcat is 1038 (s-1) with UDP- GalNAc. {ECO:0000269|PubMed:12484781}; pH dependence: Optimum pH is 7.0-9.0. {ECO:0000269|PubMed:12484781}; Temperature dependence: Optimum temperature is 37 degrees Celsius. {ECO:0000269|PubMed:12484781};
Pathway:		Bacterial outer membrane biogenesis; LPS O-antigen biosynthesis. {ECO:0000303|PubMed:12484781, ECO:0000303|PubMed:21384454, ECO:0000305}.
Subunit:		Homodimer. {ECO:0000269|PubMed:12484781, ECO:0000269|PubMed:21384454}.
Similarity:		Belongs to the NAD(P)-dependent epimerase/dehydratase family. {ECO:0000305}.

Annotations taken from UniProtKB at the EBI.