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UniProt functional annotation for Q04416 | ||
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| UniProt code: Q04416. |
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| Organism: | |
Arthrobacter sp. |
| Taxonomy: | |
Bacteria; Actinobacteria; Micrococcales; Micrococcaceae; Arthrobacter. |
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| Catalytic activity: | |
Reaction=4-hydroxybenzoyl-CoA + H2O = 4-hydroxybenzoate + CoA + H(+); Xref=Rhea:RHEA:11948, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17879, ChEBI:CHEBI:57287, ChEBI:CHEBI:57356; EC=3.1.2.23; Evidence={ECO:0000269|PubMed:12732540}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=243 uM for benzoyl-CoA {ECO:0000269|PubMed:12732540}; KM=1.24 uM for 4-hydroxybenzoyl-CoA {ECO:0000269|PubMed:12732540}; KM=22 uM for 3-hydroxybenzoyl-CoA {ECO:0000269|PubMed:12732540}; KM=29 uM for 2,5-dihydroxybenzoyl-CoA {ECO:0000269|PubMed:12732540}; KM=113 uM for 4-chlorobenzoyl-CoA {ECO:0000269|PubMed:12732540}; KM=140 uM for phenylacetyl-CoA {ECO:0000269|PubMed:12732540}; KM=2200 uM for acetyl-CoA {ECO:0000269|PubMed:12732540}; KM=1100 uM for propionyl-CoA {ECO:0000269|PubMed:12732540}; KM=930 uM for butyryl-CoA {ECO:0000269|PubMed:12732540}; KM=810 uM for crotonyl-CoA {ECO:0000269|PubMed:12732540}; pH dependence: Optimum pH is 4.6-9.6. {ECO:0000269|PubMed:12732540}; |
| Pathway: | |
Xenobiotic degradation; 4-chlorobenzoate degradation; 4- hydroxybenzoate from 4-chlorobenzoate: step 3/3. |
| Subunit: | |
Homotetramer. {ECO:0000269|PubMed:12907670}. |
| Mass spectrometry: | |
Mass=16394.40; Method=Electrospray; Evidence={ECO:0000269|PubMed:12732540}; |
| Similarity: | |
Belongs to the thioesterase PaaI family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.