UniProt functional annotation for Q7QIJ8



	UniProt functional annotation for Q7QIJ8

UniProt code: Q7QIJ8.

Organism: Anopheles gambiae (African malaria mosquito).

Taxonomy: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Nematocera; Culicoidea; Culicidae; Anophelinae; Anopheles.

Function: Serine protease inhibitor that functions in the melanization- mediated immune response (PubMed:16113656, PubMed:20953892, PubMed:21465556, PubMed:25525260). By preventing the activation of phenoloxidases through the inhibiting of serine peptidase CLIPB9, negatively regulates melanization in the hemolymph (PubMed:16113656, PubMed:20953892, PubMed:21465556, PubMed:25525260). By preventing melanization, has a detrimental role during P.berghei parasite mediated-infection and invasion of the mosquito midgut (PubMed:16113656). {ECO:0000269|PubMed:16113656, ECO:0000269|PubMed:20953892, ECO:0000269|PubMed:21465556, ECO:0000269|PubMed:25525260}.

Subunit: Forms a covalent heterodimer with peptidase CLIPB9; the interaction inhibits CLIPB9 peptidase activity. {ECO:0000269|PubMed:20953892, ECO:0000269|PubMed:25525260}.

Subcellular location: Secreted {ECO:0000269|PubMed:16113656}. Note=Secreted into the hemolymph. {ECO:0000269|PubMed:16113656, ECO:0000269|PubMed:20953892}.

Developmental stage: Expressed in embryos, larvae, pupae, and female and male adults. {ECO:0000269|PubMed:19394412}.

Ptm: PPeptidase CLIPB9 binds to SRPN2 via the hinge region resulting in the cleavage of the reactive bond (PubMed:25525260). This leads to a conformational change in SRPN2 which traps CLIPB9 and distorts its active site, resulting in CLIPB9 inactivation (PubMed:25525260). {ECO:0000269|PubMed:25525260}.

Disruption phenotype: RNAi-mediated knockdown in female causes spontaneous melanization leading to the formation of melanotic pseudotumors and a shortening of lifespan (PubMed:16113656, PubMed:20953892). Simultaneous RNAi-mediated knockdown of SRPN2 and CLIPB9, reduces the spontaneous melanization and the lifespan shortening (PubMed:20953892). During the mosquito midgut infection by the rodent malaria parasite P.berghei, severely reduces oocyst numbers due to an increase in ookinete lysis and melanization (PubMed:16113656). Does not affect ookinete formation (PubMed:16113656). {ECO:0000269|PubMed:16113656, ECO:0000269|PubMed:20953892}.

Similarity: Belongs to the serpin family. {ECO:0000255|RuleBase:RU000411}.

Annotations taken from UniProtKB at the EBI.


Organism:		Anopheles gambiae (African malaria mosquito).
Taxonomy:		Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Nematocera; Culicoidea; Culicidae; Anophelinae; Anopheles.



Function:		Serine protease inhibitor that functions in the melanization- mediated immune response (PubMed:16113656, PubMed:20953892, PubMed:21465556, PubMed:25525260). By preventing the activation of phenoloxidases through the inhibiting of serine peptidase CLIPB9, negatively regulates melanization in the hemolymph (PubMed:16113656, PubMed:20953892, PubMed:21465556, PubMed:25525260). By preventing melanization, has a detrimental role during P.berghei parasite mediated-infection and invasion of the mosquito midgut (PubMed:16113656). {ECO:0000269\|PubMed:16113656, ECO:0000269\|PubMed:20953892, ECO:0000269\|PubMed:21465556, ECO:0000269\|PubMed:25525260}.


Subunit:		Forms a covalent heterodimer with peptidase CLIPB9; the interaction inhibits CLIPB9 peptidase activity. {ECO:0000269\|PubMed:20953892, ECO:0000269\|PubMed:25525260}.
Subcellular location:		Secreted {ECO:0000269\|PubMed:16113656}. Note=Secreted into the hemolymph. {ECO:0000269\|PubMed:16113656, ECO:0000269\|PubMed:20953892}.
Developmental stage:		Expressed in embryos, larvae, pupae, and female and male adults. {ECO:0000269\|PubMed:19394412}.
Ptm:		PPeptidase CLIPB9 binds to SRPN2 via the hinge region resulting in the cleavage of the reactive bond (PubMed:25525260). This leads to a conformational change in SRPN2 which traps CLIPB9 and distorts its active site, resulting in CLIPB9 inactivation (PubMed:25525260). {ECO:0000269\|PubMed:25525260}.
Disruption phenotype:		RNAi-mediated knockdown in female causes spontaneous melanization leading to the formation of melanotic pseudotumors and a shortening of lifespan (PubMed:16113656, PubMed:20953892). Simultaneous RNAi-mediated knockdown of SRPN2 and CLIPB9, reduces the spontaneous melanization and the lifespan shortening (PubMed:20953892). During the mosquito midgut infection by the rodent malaria parasite P.berghei, severely reduces oocyst numbers due to an increase in ookinete lysis and melanization (PubMed:16113656). Does not affect ookinete formation (PubMed:16113656). {ECO:0000269\|PubMed:16113656, ECO:0000269\|PubMed:20953892}.
Similarity:		Belongs to the serpin family. {ECO:0000255\|RuleBase:RU000411}.