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PDBsum entry 3psr
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Ef-hand protein
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PDB id
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3psr
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Contents |
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* Residue conservation analysis
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References listed in PDB file
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Key reference
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Title
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Zinc-Binding site of an s100 protein revealed. Two crystal structures of ca2+-Bound human psoriasin (s100a7) in the zn2+-Loaded and zn2+-Free states.
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Authors
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D.E.Brodersen,
J.Nyborg,
M.Kjeldgaard.
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Ref.
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Biochemistry, 1999,
38,
1695-1704.
[DOI no: ]
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PubMed id
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Abstract
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The crystal structure of human psoriasin (S100A7) in the native, calcium-bound
form has been determined from two crystal forms of the protein crystallized with
and without divalent zinc. The overall structures of the dimeric protein closely
resemble the previously determined holmium-substituted structure. The structures
also reveal a zinc-binding site of the protein, which is formed by three
histidines and an aspartate residue. Together, these residues coordinate the
zinc ion in a way similar to the pattern seen in certain metalloproteases and in
particular the collagenase family of proteins. Sequence comparison suggests that
this zinc site is present in a number of the remaining members of the S100
family. The structure of S100A7 crystallized in the absence of zinc further
shows that loss of zinc results in a reorganization of the adjacent empty and
distorted EF-hand loop, causing it to resemble a calcium-loaded EF-hand.
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Secondary reference #1
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Title
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Ef-Hands at atomic resolution: the structure of human psoriasin (s100a7) solved by mad phasing.
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Authors
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D.E.Brodersen,
M.Etzerodt,
P.Madsen,
J.E.Celis,
H.C.Thøgersen,
J.Nyborg,
M.Kjeldgaard.
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Ref.
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Structure, 1998,
6,
477-489.
[DOI no: ]
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PubMed id
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Figure 7.
Figure 7. The Ramachandran plot for the final model.
Non-glycine and non-proline residues are indicated by solid
circles (asparagine residues as solid diamonds) and glycine
residues as open squares. The shaded area corresponds to the 98%
core regions as defined by Kleywegt and Jones [38]. Plot was
created with rama (MK, unpublished program).
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The above figure is
reproduced from the cited reference
with permission from Cell Press
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Secondary reference #2
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Title
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Crystallization and preliminary X-Ray diffraction studies of psoriasin.
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Authors
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S.Nolsøe,
S.Thirup,
M.Etzerodt,
H.C.Thøgersen,
J.Nyborg.
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Ref.
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Acta Crystallogr D Biol Crystallogr, 1997,
53,
119-121.
[DOI no: ]
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PubMed id
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Figure 1.
Fig. 1. (a) Crystal form I. Crystals of psoriasin, crystallized in the
presence of Ca2+with dimensions 500 x 50 × 50 ~tm. (b) Crystal
form II. Crystals of psoriasin, crystallized in the presence of Zn
2+
with dimensions 100 x 100 x 200 lam.
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The above figure is
reproduced from the cited reference
with permission from the IUCr
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Secondary reference #3
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Title
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Molecular cloning, Occurrence, And expression of a novel partially secreted protein "psoriasin" that is highly up-Regulated in psoriatic skin.
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Authors
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P.Madsen,
H.H.Rasmussen,
H.Leffers,
B.Honoré,
K.Dejgaard,
E.Olsen,
J.Kiil,
E.Walbum,
A.H.Andersen,
B.Basse.
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Ref.
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J Invest Dermatol, 1991,
97,
701-712.
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PubMed id
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