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PDBsum entry 3php
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References listed in PDB file
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Key reference
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Title
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Structure of the 3'-Hairpin of the tymv pseudoknot: preformation in RNA folding.
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Authors
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M.H.Kolk,
M.Van der graaf,
C.T.Fransen,
S.S.Wijmenga,
C.W.Pleij,
H.A.Heus,
C.W.Hilbers.
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Ref.
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Embo J, 1998,
17,
7498-7504.
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PubMed id
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Abstract
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The solution structure of an RNA-hairpin present in the pseudoknot, which is
found at the 3'-terminus of turnip yellow mosaic virus genomic RNA, has been
solved by nuclear magnetic resonance spectroscopy. The loop, which contains the
sequence 5'-GGGUCA-3', was found to be highly structured and, contrary to
expectations, does not attain its stability through GA or GC base pair formation
but by triple interactions between the tilted adenosine and the minor groove
sides of the first two guanosines. Interestingly, a very similar conformation
was found for the cognate pseudoknot, implying that the 3'-hairpin is preformed
for folding into a pseudoknotted structure. These findings suggest a mechanism
of 'predetermined-fit' as a principle in RNA folding.
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Headers
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