PDBsum entry 3ouc

Hydrolase/peptide

PDB id: 3ouc

Contents

Protein chains

99 a.a.

Ligands

THR-ILE-MET-MET-GLN-ARG-GLY

Waters ×191

PDB id:

3ouc

Name:

Hydrolase/peptide

Title:

Mdr769 HIV-1 protease complexed with p2/nc hepta-peptide

Structure:

Mdr HIV-1 protease. Chain: a, b. Engineered: yes. P2/nc substrate peptide. Chain: p. Engineered: yes

Source:

Human immunodeficiency virus 1. Organism_taxid: 11676. Gene: pol. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. HIV-1. Other_details: this sequence occurs naturally in HIV-1

Resolution:

2.00Å R-factor: 0.199 R-free: 0.262

Authors:

Z.Liu,Y.Wang,J.Brunzelle,I.A.Kovari,L.C.Kovari

Key ref:

Z.Liu et al. (2011). Nine crystal structures determine the substrate envelope of the MDR HIV-1 protease. Protein J, 30, 173-183. PubMed id: 21394574

Date:

14-Sep-10 Release date: 30-Mar-11

Protein chains

P35963  (POL_HV1Y2) -  Gag-Pol polyprotein from Human immunodeficiency virus type 1 group M subtype B (isolate YU-2)

Seq: 1435 a.a.

Struc: 99 a.a.*

* PDB and UniProt seqs differ at 10 residue positions (black crosses)

Enzyme reactions

• Enzyme class 1: E.C.2.7.7.- - ?????
• Enzyme class 2: E.C.2.7.7.49 - RNA-directed Dna polymerase.
• Reaction: DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate
• Enzyme class 3: E.C.2.7.7.7 - DNA-directed Dna polymerase.
• Reaction: DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate
• Enzyme class 4: E.C.3.1.-.-
• Enzyme class 5: E.C.3.1.13.2 - exoribonuclease H.
• Reaction: Exonucleolytic cleavage to 5'-phosphomonoester oligonucleotides in both 5'- to 3'- and 3'- to 5'-directions.
• Enzyme class 6: E.C.3.1.26.13 - retroviral ribonuclease H.
• Enzyme class 7: E.C.3.4.23.16 - HIV-1 retropepsin.
• Reaction: Specific for a P1 residue that is hydrophobic, and P1' variable, but often Pro.

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

Protein J 30:173-183 (2011)

PubMed id: 21394574

Nine crystal structures determine the substrate envelope of the MDR HIV-1 protease.

Z.Liu, Y.Wang, J.Brunzelle, I.A.Kovari, L.C.Kovari.

ABSTRACT

No abstract given.