PDBsum entry 3oj7

Metal binding protein

PDB id: 3oj7

Contents

Protein chain

109 a.a. *

Ligands

SO4

Metals

_ZN

Waters ×98

* Residue conservation analysis

PDB id:

3oj7

Name:

Metal binding protein

Title:

Crystal structure of a histidine triad family protein from entamoeba histolytica, bound to sulfate

Structure:

Putative histidine triad family protein. Chain: a. Engineered: yes

Source:

Entamoeba histolytica. Organism_taxid: 294381. Strain: hm-1:imss. Gene: ehi_093910. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

1.40Å R-factor: 0.163 R-free: 0.184

Authors:

Seattle Structural Genomics Center For Infectious Disease (Ssgcid)

Key ref:

D.D.Lorimer et al. (2015). Structures of a histidine triad family protein from Entamoeba histolytica bound to sulfate, AMP and GMP. Acta Crystallogr F Struct Biol Commun, 71, 572-576. PubMed id: 25945711 DOI: 10.1107/S2053230X1500237X

Date:

20-Aug-10 Release date: 08-Sep-10

Protein chain

C4LYI2  (HIT_ENTHI) -  Histidine triad nucleotide-binding protein from Entamoeba histolytica (strain ATCC 30459 / HM-1:IMSS / ABRM)

Seq: 113 a.a.

Struc: 109 a.a.

Enzyme reactions

• Enzyme class: E.C.3.9.1.- - ?????

DOI no: 10.1107/S2053230X1500237X

Acta Crystallogr F Struct Biol Commun 71:572-576 (2015)

PubMed id: 25945711

Structures of a histidine triad family protein from Entamoeba histolytica bound to sulfate, AMP and GMP.

D.D.Lorimer, R.Choi, A.Abramov, S.Nakazawa Hewitt, A.S.Gardberg, W.C.Van Voorhis, B.L.Staker, P.J.Myler, T.E.Edwards.

ABSTRACT

Three structures of the histidine triad family protein from Entamoeba histolytica, the causative agent of amoebic dysentery, were solved at high resolution within the Seattle Structural Genomics Center for Infectious Disease (SSGCID). The structures have sulfate (PDB entry 3oj7), AMP (PDB entry 3omf) or GMP (PDB entry 3oxk) bound in the active site, with sulfate occupying the same space as the α-phosphate of the two nucleotides. The C(α) backbones of the three structures are nearly superimposable, with pairwise r.m.s.d.s ranging from 0.06 to 0.13 Å.