UniProt functional annotation for Q39255



	UniProt functional annotation for Q39255

UniProt code: Q39255.

Organism: Arabidopsis thaliana (Mouse-ear cress).

Taxonomy: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.

Function: Involved in ubiquitination and subsequent proteasomal degradation of target proteins. Together with CUL1, RBX1 and a F-box protein, it forms a SCF E3 ubiquitin ligase complex. The functional specificity of this complex depends on the type of F-box protein. In the SCF complex, it serves as an adapter that links the F-box protein to CUL1. SCF(UFO) is required for vegetative and floral organ development as well as for male gametogenesis. SCF(TIR1) is involved in auxin signaling pathway. SCF(COI1) regulates responses to jasmonates. SCF(EID1) and SCF(AFR) are implicated in phytochrome A light signaling. SCF(ADO1), SCF(ADO2), SCF(ADO3) are related to the circadian clock. SCF(ORE9) seems to be involved in senescence. SCF(EBF1/EBF2) may regulate ethylene signaling. Plays a role during embryogenesis and early postembryonic development, especially during cell elongation and division. Contributes to the correct chromosome segregation during tetrad formation. {ECO:0000269|PubMed:10398681, ECO:0000269|PubMed:10500191, ECO:0000269|PubMed:10528262, ECO:0000269|PubMed:11526079, ECO:0000269|PubMed:12970487, ECO:0000269|PubMed:14688296}.

Pathway: Protein modification; protein ubiquitination.

Subunit: Part of a SCF E3 ubiquitin ligase complex composed of SKP1, CUL1, RBX1 (RBX1A or RBX1B) and F-box proteins. Interacts with SKIP1, SKIP2, SKIP3, SKIP4, SKIP6, FIB1/SKIP7, SKIP8, PP2A11/SKIP10, SKIP11, PP2B11/SKIP12, PP2A14/SKIP13, SKIP14, SKIP15, SKIP16, SKIP19/FBL20, SKIP20, PP2B1/SKIP21, SKIP22, SKIP23, SKIP24, SKIP25, TULP10/SKIP26, SKIP27, SKIP28/MEE11, AFR/SKIP29, SKIP30, SKIP31, SKIP32/FBP7, SKIP33, SKIP35, ADO1/ZTL, ADO2/LKP2, ADO3/FKF1, AFR, COI1, DOR, EBF1, EBF2, EID1, ORE9, PP2A13/SKIP9, TIR1, UFO, SKP2A, CPR1/CPR30, FBL17, NUP58, At1g55000, At1g67340, At1g78100, At3g04660, At3g61590, At4g38940 and At5g49610. The SKP1A subunit of the SCF E3 ubiquitin ligase complex can interact directly with KIN10, KIN11 and the proteasome subunit PAD1. This interaction can be disrupted by PRL1. In case of polerovirus infection, part of a SCF P0 complex composed of the viral silencing suppressor P0, SKP1 and CUL1. Interacts with turnip yellows virus P0. Interacts with VBF and Agrobacterium virF. Binds to KIB1 (PubMed:28575660). {ECO:0000269|PubMed:10398681, ECO:0000269|PubMed:10607296, ECO:0000269|PubMed:11316788, ECO:0000269|PubMed:11387208, ECO:0000269|PubMed:11487692, ECO:0000269|PubMed:12169662, ECO:0000269|PubMed:12172031, ECO:0000269|PubMed:12381738, ECO:0000269|PubMed:12445118, ECO:0000269|PubMed:12795696, ECO:0000269|PubMed:12826617, ECO:0000269|PubMed:14653999, ECO:0000269|PubMed:14675532, ECO:0000269|PubMed:14749489, ECO:0000269|PubMed:15310821, ECO:0000269|PubMed:15343337, ECO:0000269|PubMed:15447654, ECO:0000269|PubMed:16446454, ECO:0000269|PubMed:17410169, ECO:0000269|PubMed:18036202, ECO:0000269|PubMed:18391211, ECO:0000269|PubMed:18835996, ECO:0000269|PubMed:18948957, ECO:0000269|PubMed:19682297, ECO:0000269|PubMed:20227663, ECO:0000269|PubMed:20927106, ECO:0000269|PubMed:23840761, ECO:0000269|PubMed:28575660}.

Subcellular location: Nucleus {ECO:0000269|PubMed:11387208}. Cytoplasm, cytoskeleton, spindle {ECO:0000269|PubMed:11387208}. Cytoplasm, cytoskeleton, phragmoplast {ECO:0000269|PubMed:11387208}. Note=Associated to mitotic spindle and phragmoplasts during cell division.

Tissue specificity: Accumulates only in meristematic cells. Expressed in inflorescence, shoot and root apical meristems, as well as in developing organs such as gametocytes and seeds. Also detected in cortical layer and epidermis of roots, leaves, pith and vascular bundle of young stem, young floral buds and organ primordia, pollen and through the valve of siliques. Not detectable in mature root tissues. {ECO:0000269|PubMed:10607296, ECO:0000269|PubMed:12970487, ECO:0000269|PubMed:14688296, ECO:0000269|PubMed:14749489, ECO:0000269|PubMed:9530878}.

Developmental stage: During flower development, expressed in the tapetal layer surrounding the male microsporocytes, and in the endothelium layer surrounding the embryo sac within the ovule. During embryogenesis, expressed in all cells of the embryo and in developing endosperm surrounding the embryo, at the time of free nuclei proliferation. {ECO:0000269|PubMed:9530878}.

Similarity: Belongs to the SKP1 family. {ECO:0000305}.

Sequence caution: Sequence=AAB38862.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};

Annotations taken from UniProtKB at the EBI.


Organism:		Arabidopsis thaliana (Mouse-ear cress).
Taxonomy:		Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.



Function:		Involved in ubiquitination and subsequent proteasomal degradation of target proteins. Together with CUL1, RBX1 and a F-box protein, it forms a SCF E3 ubiquitin ligase complex. The functional specificity of this complex depends on the type of F-box protein. In the SCF complex, it serves as an adapter that links the F-box protein to CUL1. SCF(UFO) is required for vegetative and floral organ development as well as for male gametogenesis. SCF(TIR1) is involved in auxin signaling pathway. SCF(COI1) regulates responses to jasmonates. SCF(EID1) and SCF(AFR) are implicated in phytochrome A light signaling. SCF(ADO1), SCF(ADO2), SCF(ADO3) are related to the circadian clock. SCF(ORE9) seems to be involved in senescence. SCF(EBF1/EBF2) may regulate ethylene signaling. Plays a role during embryogenesis and early postembryonic development, especially during cell elongation and division. Contributes to the correct chromosome segregation during tetrad formation. {ECO:0000269\|PubMed:10398681, ECO:0000269\|PubMed:10500191, ECO:0000269\|PubMed:10528262, ECO:0000269\|PubMed:11526079, ECO:0000269\|PubMed:12970487, ECO:0000269\|PubMed:14688296}.


Pathway:		Protein modification; protein ubiquitination.
Subunit:		Part of a SCF E3 ubiquitin ligase complex composed of SKP1, CUL1, RBX1 (RBX1A or RBX1B) and F-box proteins. Interacts with SKIP1, SKIP2, SKIP3, SKIP4, SKIP6, FIB1/SKIP7, SKIP8, PP2A11/SKIP10, SKIP11, PP2B11/SKIP12, PP2A14/SKIP13, SKIP14, SKIP15, SKIP16, SKIP19/FBL20, SKIP20, PP2B1/SKIP21, SKIP22, SKIP23, SKIP24, SKIP25, TULP10/SKIP26, SKIP27, SKIP28/MEE11, AFR/SKIP29, SKIP30, SKIP31, SKIP32/FBP7, SKIP33, SKIP35, ADO1/ZTL, ADO2/LKP2, ADO3/FKF1, AFR, COI1, DOR, EBF1, EBF2, EID1, ORE9, PP2A13/SKIP9, TIR1, UFO, SKP2A, CPR1/CPR30, FBL17, NUP58, At1g55000, At1g67340, At1g78100, At3g04660, At3g61590, At4g38940 and At5g49610. The SKP1A subunit of the SCF E3 ubiquitin ligase complex can interact directly with KIN10, KIN11 and the proteasome subunit PAD1. This interaction can be disrupted by PRL1. In case of polerovirus infection, part of a SCF P0 complex composed of the viral silencing suppressor P0, SKP1 and CUL1. Interacts with turnip yellows virus P0. Interacts with VBF and Agrobacterium virF. Binds to KIB1 (PubMed:28575660). {ECO:0000269\|PubMed:10398681, ECO:0000269\|PubMed:10607296, ECO:0000269\|PubMed:11316788, ECO:0000269\|PubMed:11387208, ECO:0000269\|PubMed:11487692, ECO:0000269\|PubMed:12169662, ECO:0000269\|PubMed:12172031, ECO:0000269\|PubMed:12381738, ECO:0000269\|PubMed:12445118, ECO:0000269\|PubMed:12795696, ECO:0000269\|PubMed:12826617, ECO:0000269\|PubMed:14653999, ECO:0000269\|PubMed:14675532, ECO:0000269\|PubMed:14749489, ECO:0000269\|PubMed:15310821, ECO:0000269\|PubMed:15343337, ECO:0000269\|PubMed:15447654, ECO:0000269\|PubMed:16446454, ECO:0000269\|PubMed:17410169, ECO:0000269\|PubMed:18036202, ECO:0000269\|PubMed:18391211, ECO:0000269\|PubMed:18835996, ECO:0000269\|PubMed:18948957, ECO:0000269\|PubMed:19682297, ECO:0000269\|PubMed:20227663, ECO:0000269\|PubMed:20927106, ECO:0000269\|PubMed:23840761, ECO:0000269\|PubMed:28575660}.
Subcellular location:		Nucleus {ECO:0000269\|PubMed:11387208}. Cytoplasm, cytoskeleton, spindle {ECO:0000269\|PubMed:11387208}. Cytoplasm, cytoskeleton, phragmoplast {ECO:0000269\|PubMed:11387208}. Note=Associated to mitotic spindle and phragmoplasts during cell division.
Tissue specificity:		Accumulates only in meristematic cells. Expressed in inflorescence, shoot and root apical meristems, as well as in developing organs such as gametocytes and seeds. Also detected in cortical layer and epidermis of roots, leaves, pith and vascular bundle of young stem, young floral buds and organ primordia, pollen and through the valve of siliques. Not detectable in mature root tissues. {ECO:0000269\|PubMed:10607296, ECO:0000269\|PubMed:12970487, ECO:0000269\|PubMed:14688296, ECO:0000269\|PubMed:14749489, ECO:0000269\|PubMed:9530878}.
Developmental stage:		During flower development, expressed in the tapetal layer surrounding the male microsporocytes, and in the endothelium layer surrounding the embryo sac within the ovule. During embryogenesis, expressed in all cells of the embryo and in developing endosperm surrounding the embryo, at the time of free nuclei proliferation. {ECO:0000269\|PubMed:9530878}.
Similarity:		Belongs to the SKP1 family. {ECO:0000305}.
Sequence caution:		Sequence=AAB38862.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};