UniProt functional annotation for Q5SHN3



	UniProt functional annotation for Q5SHN3

UniProt code: Q5SHN3.

Organism: Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8).

Taxonomy: Bacteria; Deinococcus-Thermus; Deinococci; Thermales; Thermaceae; Thermus.

Function: With S4 and S5 plays an important role in translational accuracy. {ECO:0000250}.

Function: Interacts with and stabilizes bases of the 16S rRNA that are involved in tRNA selection in the A site and with the mRNA backbone (PubMed:11014182, PubMed:11007480, PubMed:11163189, PubMed:11014183, PubMed:11511350, PubMed:11296217, PubMed:11228145, PubMed:11228145, PubMed:11283358, PubMed:11340196, PubMed:11866529). Located at the interface of the 30S and 50S subunits, it traverses the body of the 30S subunit contacting proteins on the other side and probably holding the rRNA structure together. The combined cluster of proteins S8, S12 and S17 appears to hold together the shoulder and platform of the 30S subunit (PubMed:11283358). {ECO:0000269|PubMed:11007480, ECO:0000269|PubMed:11014182, ECO:0000269|PubMed:11014183, ECO:0000269|PubMed:11163189, ECO:0000269|PubMed:11228145, ECO:0000269|PubMed:11283358, ECO:0000269|PubMed:11296217, ECO:0000269|PubMed:11340196, ECO:0000269|PubMed:11511350, ECO:0000269|PubMed:11866529}.

Subunit: Part of the 30S ribosomal subunit (PubMed:11014182, PubMed:11007480, PubMed:11163189, PubMed:11014183, PubMed:11511350, PubMed:11296217, PubMed:11228145, PubMed:11228145, PubMed:11283358, PubMed:11340196, PubMed:11866529, PubMed:25775268, PubMed:26792896). Contacts proteins S8 and S17 (PubMed:11866529). Interacts with IF1 in the 30S initiation complex (PubMed:11228145). {ECO:0000269|PubMed:11007480, ECO:0000269|PubMed:11163189, ECO:0000269|PubMed:11228145, ECO:0000269|PubMed:11283358, ECO:0000269|PubMed:11296217, ECO:0000269|PubMed:11340196, ECO:0000269|PubMed:11511350, ECO:0000269|PubMed:11866529, ECO:0000269|PubMed:25775268, ECO:0000269|PubMed:26792896}.

Ptm: The methythiolated Asp-89 contacts a modifed residue of the 16S rRNA close to the decoding center, and might be important for decoding center assembly. {ECO:0000269|PubMed:25775268}.

Mass spectrometry: Mass=14516; Method=MALDI; Evidence={ECO:0000269|PubMed:16287167};

Similarity: Belongs to the universal ribosomal protein uS12 family. {ECO:0000305}.

Sequence caution: Sequence=BAD71520.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305}; Sequence=CAA36418.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};

Annotations taken from UniProtKB at the EBI.


Organism:		Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8).
Taxonomy:		Bacteria; Deinococcus-Thermus; Deinococci; Thermales; Thermaceae; Thermus.



Function:		With S4 and S5 plays an important role in translational accuracy. {ECO:0000250}.



Function:		Interacts with and stabilizes bases of the 16S rRNA that are involved in tRNA selection in the A site and with the mRNA backbone (PubMed:11014182, PubMed:11007480, PubMed:11163189, PubMed:11014183, PubMed:11511350, PubMed:11296217, PubMed:11228145, PubMed:11228145, PubMed:11283358, PubMed:11340196, PubMed:11866529). Located at the interface of the 30S and 50S subunits, it traverses the body of the 30S subunit contacting proteins on the other side and probably holding the rRNA structure together. The combined cluster of proteins S8, S12 and S17 appears to hold together the shoulder and platform of the 30S subunit (PubMed:11283358). {ECO:0000269\|PubMed:11007480, ECO:0000269\|PubMed:11014182, ECO:0000269\|PubMed:11014183, ECO:0000269\|PubMed:11163189, ECO:0000269\|PubMed:11228145, ECO:0000269\|PubMed:11283358, ECO:0000269\|PubMed:11296217, ECO:0000269\|PubMed:11340196, ECO:0000269\|PubMed:11511350, ECO:0000269\|PubMed:11866529}.


Subunit:		Part of the 30S ribosomal subunit (PubMed:11014182, PubMed:11007480, PubMed:11163189, PubMed:11014183, PubMed:11511350, PubMed:11296217, PubMed:11228145, PubMed:11228145, PubMed:11283358, PubMed:11340196, PubMed:11866529, PubMed:25775268, PubMed:26792896). Contacts proteins S8 and S17 (PubMed:11866529). Interacts with IF1 in the 30S initiation complex (PubMed:11228145). {ECO:0000269\|PubMed:11007480, ECO:0000269\|PubMed:11163189, ECO:0000269\|PubMed:11228145, ECO:0000269\|PubMed:11283358, ECO:0000269\|PubMed:11296217, ECO:0000269\|PubMed:11340196, ECO:0000269\|PubMed:11511350, ECO:0000269\|PubMed:11866529, ECO:0000269\|PubMed:25775268, ECO:0000269\|PubMed:26792896}.
Ptm:		The methythiolated Asp-89 contacts a modifed residue of the 16S rRNA close to the decoding center, and might be important for decoding center assembly. {ECO:0000269\|PubMed:25775268}.
Mass spectrometry:		Mass=14516; Method=MALDI; Evidence={ECO:0000269\|PubMed:16287167};
Similarity:		Belongs to the universal ribosomal protein uS12 family. {ECO:0000305}.
Sequence caution:		Sequence=BAD71520.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305}; Sequence=CAA36418.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};