UniProt functional annotation for Q12018



	UniProt functional annotation for Q12018

UniProt code: Q12018.

Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).

Taxonomy: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.

Function: Core component of multiple cullin-RING-based SCF (SKP1-CUL1- F-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The SCF complex associates with CDC34 as the E2 ubiquitin-conjugating enzyme. The functional specificity of the SCF complex depends on the type of F-box protein. SCF(CDC4) controls the G1-to-S phase transition; it directs ubiquitination of the phosphorylated CDK inhibitor SIC1 and of CDC6. SCF(CDC4) directs ubiquitination of GCN4. SCF(GRR1) directs ubiquitination of phosphorylated CLN1, CLN2 and GIC2. SCF(MET30) directs ubiquitination of MET4. SCF(DIA2) is specifically involved in the pheromone induced degradation of phosphorylated TEC1. SCF(MDM30) seems to direct ubiquitination of FZ01. Involved in the regulation of methionine biosynthesis genes. {ECO:0000269|PubMed:10213692, ECO:0000269|PubMed:16421250, ECO:0000269|PubMed:7813440, ECO:0000269|PubMed:9312054, ECO:0000269|PubMed:9346238, ECO:0000269|PubMed:9346239, ECO:0000269|PubMed:9499404, ECO:0000269|PubMed:9736614}.

Subunit: Component of multiple SCF (SKP1-CUL1-F-box) E3 ubiquitin- protein ligase complexes formed of CUL1, SKP1/HRT1, RBX1 and a variable F-box domain-containing protein as substrate-specific adapter. Component of the SCF(CDC4) complex containing CDC4. Component of the SCF(MET30) complex containing MET30. Component of the SCF(GRR1) complex containig GRR1. Component of the probable SCF(DIA2) complex containing DIA2. Component of the probable SCF(YDR131C) complex containing YDR131C. Component of the probable SCF(YDR306C) complex containing YDR306C. Component of the probable SCF(YLR224W) complex containing YLR224W. Component of the probable SCF(YJL149W) complex containing YJL149W. Component of the probable SCF(YNL311C) complex containing YNL311C. Component of the probable SCF(MDM30) complex containing MDM30. Component of the probable SCF(UFO1) complex containing UFO1. Component of the probable SCF(HRT3) complex containing HRT3. Component of the probable SCF(YBR280C) complex containing YBR280C. Component of the probable SCF(YBR352W) complex containing YBR352W. Interacts with DCN1, YBR280C, YLR224W and YLR352W. The unneddylated form interacts with LAG2/CAND1 and the interaction mediates the exchange of the F-box substrate-specific subunit. {ECO:0000269|PubMed:10385629, ECO:0000269|PubMed:11283612, ECO:0000269|PubMed:16421250, ECO:0000269|PubMed:19763088, ECO:0000269|PubMed:19942853, ECO:0000269|PubMed:9346238, ECO:0000269|PubMed:9346239, ECO:0000269|PubMed:9499404}.

Subcellular location: Cytoplasm {ECO:0000269|PubMed:14562095}. Nucleus {ECO:0000269|PubMed:14562095}.

Ptm: Neddylated; enhancing the ubiquitin-ligase activity. {ECO:0000250|UniProtKB:P47050}.

Miscellaneous: Present with 377 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}.

Similarity: Belongs to the cullin family. {ECO:0000255|PROSITE- ProRule:PRU00330}.

Annotations taken from UniProtKB at the EBI.


Organism:		Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
Taxonomy:		Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.



Function:		Core component of multiple cullin-RING-based SCF (SKP1-CUL1- F-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The SCF complex associates with CDC34 as the E2 ubiquitin-conjugating enzyme. The functional specificity of the SCF complex depends on the type of F-box protein. SCF(CDC4) controls the G1-to-S phase transition; it directs ubiquitination of the phosphorylated CDK inhibitor SIC1 and of CDC6. SCF(CDC4) directs ubiquitination of GCN4. SCF(GRR1) directs ubiquitination of phosphorylated CLN1, CLN2 and GIC2. SCF(MET30) directs ubiquitination of MET4. SCF(DIA2) is specifically involved in the pheromone induced degradation of phosphorylated TEC1. SCF(MDM30) seems to direct ubiquitination of FZ01. Involved in the regulation of methionine biosynthesis genes. {ECO:0000269\|PubMed:10213692, ECO:0000269\|PubMed:16421250, ECO:0000269\|PubMed:7813440, ECO:0000269\|PubMed:9312054, ECO:0000269\|PubMed:9346238, ECO:0000269\|PubMed:9346239, ECO:0000269\|PubMed:9499404, ECO:0000269\|PubMed:9736614}.


Subunit:		Component of multiple SCF (SKP1-CUL1-F-box) E3 ubiquitin- protein ligase complexes formed of CUL1, SKP1/HRT1, RBX1 and a variable F-box domain-containing protein as substrate-specific adapter. Component of the SCF(CDC4) complex containing CDC4. Component of the SCF(MET30) complex containing MET30. Component of the SCF(GRR1) complex containig GRR1. Component of the probable SCF(DIA2) complex containing DIA2. Component of the probable SCF(YDR131C) complex containing YDR131C. Component of the probable SCF(YDR306C) complex containing YDR306C. Component of the probable SCF(YLR224W) complex containing YLR224W. Component of the probable SCF(YJL149W) complex containing YJL149W. Component of the probable SCF(YNL311C) complex containing YNL311C. Component of the probable SCF(MDM30) complex containing MDM30. Component of the probable SCF(UFO1) complex containing UFO1. Component of the probable SCF(HRT3) complex containing HRT3. Component of the probable SCF(YBR280C) complex containing YBR280C. Component of the probable SCF(YBR352W) complex containing YBR352W. Interacts with DCN1, YBR280C, YLR224W and YLR352W. The unneddylated form interacts with LAG2/CAND1 and the interaction mediates the exchange of the F-box substrate-specific subunit. {ECO:0000269\|PubMed:10385629, ECO:0000269\|PubMed:11283612, ECO:0000269\|PubMed:16421250, ECO:0000269\|PubMed:19763088, ECO:0000269\|PubMed:19942853, ECO:0000269\|PubMed:9346238, ECO:0000269\|PubMed:9346239, ECO:0000269\|PubMed:9499404}.
Subcellular location:		Cytoplasm {ECO:0000269\|PubMed:14562095}. Nucleus {ECO:0000269\|PubMed:14562095}.
Ptm:		Neddylated; enhancing the ubiquitin-ligase activity. {ECO:0000250\|UniProtKB:P47050}.
Miscellaneous:		Present with 377 molecules/cell in log phase SD medium. {ECO:0000269\|PubMed:14562106}.
Similarity:		Belongs to the cullin family. {ECO:0000255\|PROSITE- ProRule:PRU00330}.